Department of Ultrasound, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, 518107, China.
Department of orthopedics, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, 518107, China.
J Mater Sci Mater Med. 2023 Sep 21;34(10):46. doi: 10.1007/s10856-023-06749-9.
Poor selectivity, low bioavailability and serious systemic side-effects have limited the application of traditional chemotherapy method for treatment of prostate cancer. Stimuli-responsive drug delivery systems for chemotherapy are mainly based on the unique characteristics of tumor microenvironment. In this study, the GSH-sensitive poly-TTG-SS@DTX NPs (DTX-loaded poly-Tetraethylene glycol nanoparticles) were designed and synthesized, which were characterized with nanosized diameter (92.8 ± 2.5 nm) and negatively charged surface charge (-24.7 ± 5.56 mV). Experiments in vitro showed that poly-TTG-SS@DTX NPs had good compatibility to healthy cells and strong anti-tumor effect because of rapid and sustained drug release of DTX from poly-TTG-SS@DTX NPs under the tumor-microenvironment condition. The cellular activity remained greater than 90% when the concentration of poly-TTG-SS NPs reached as high as 100 µg/mL treated on healthy cells. The killing effect of DTX loading NPs group on C4-2 cells was stronger than that of free anti-tumor drug and free DTX combined with the blank nano-carrier (25.21% vs 19.93% vs 20.96%). In conclusion, poly-TTG-SS@DTX NPs may provide a new therapeutic strategy for the chemotherapy of prostate cancer.
传统的化疗方法治疗前列腺癌存在选择性差、生物利用度低和严重的全身副作用等问题，限制了其应用。用于化疗的刺激响应性药物递送系统主要基于肿瘤微环境的独特特征。在本研究中，设计并合成了 GSH 敏感的聚 TTGS@DTX NPs（载有 DTX 的聚四氢呋喃纳米颗粒），其具有纳米级直径（92.8±2.5nm）和带负电荷的表面电荷（-24.7±5.56mV）。体外实验表明，由于 DTX 在肿瘤微环境条件下从聚 TTGS@DTX NPs 中快速和持续释放，聚 TTGS@DTX NPs 具有良好的与健康细胞的相容性和较强的抗肿瘤作用。当聚 TTGS NPs 的浓度高达 100μg/mL 时，对健康细胞的细胞活性仍保持在 90%以上。与游离抗肿瘤药物和游离 DTX 与空白纳米载体的组合相比，载药 NPs 组对 C4-2 细胞的杀伤作用更强（25.21%比 19.93%比 20.96%）。总之，聚 TTGS@DTX NPs 可能为前列腺癌的化疗提供一种新的治疗策略。
