Maharjan Anoth, Park Jung-Ho
Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), Daejeon, Republic of Korea.
Biotechnol Appl Biochem. 2023 Dec;70(6):2136-2149. doi: 10.1002/bab.2514. Epub 2023 Sep 20.
Cell-free protein synthesis (CFPS) system is an innovative technology with a wide range of potential applications that could challenge current thinking and provide solutions to environmental and health issues. CFPS system has been demonstrated to be a successful way of producing biomolecules in a variety of applications, including the biomedical industry. Although there are still obstacles to overcome, its ease of use, versatility, and capacity for integration with other technologies open the door for it to continue serving as a vital instrument in synthetic biology research and industry. In this review, we mainly focus on the cell-free based platform for various product productions. Moreover, the challenges in the bio-therapeutic aspect using cell-free systems and their future prospective for the improvement and sustainability of the cell free systems.
无细胞蛋白质合成（CFPS）系统是一项具有广泛潜在应用的创新技术，它可能会挑战当前的思维方式，并为环境和健康问题提供解决方案。CFPS系统已被证明是在包括生物医学行业在内的各种应用中生产生物分子的成功方法。尽管仍有障碍需要克服，但其易用性、多功能性以及与其他技术集成的能力为其继续成为合成生物学研究和产业中的重要工具打开了大门。在本综述中，我们主要关注基于无细胞的各种产品生产平台。此外，还探讨了使用无细胞系统在生物治疗方面面临的挑战以及无细胞系统在改进和可持续发展方面的未来前景。
