Drescher Guilherme, de Vasconcelos Tassia Cristina Bello, Belo Vínicius Silva, Pinto Mariane Marques da Guarda, Rosa Jaqueline de Oliveira, Morello Luis Gustavo, Figueiredo Fabiano Borges
Cellular Biology Laboratory, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ-PR), Curitiba, Brazil.
Auditora Fiscal Federal Agropecuária do Ministério da Agricultura Pecuária e Abastecimento (MAPA), Curitiba, Brazil.
Front Vet Sci. 2023 Aug 31;10:1252454. doi: 10.3389/fvets.2023.1252454. eCollection 2023.
can cause problems in both animals and humans. Fasciolosis can be diagnosed through the indirect ELISA immunodiagnostic test. Serological diagnosis of is based on recombinant antigens secreted by this worm. We used PubMed and Google Scholar databases to review the published literature on 'antigens with immunogenic potential' used in serological tests to identify antibodies against in humans, cattle, and sheep. Studies that investigated diagnostic tests with common reference standards were included in the sensitivity and/or specificity bivariate meta-analysis. In the quality and susceptibility to bias analysis of the 33 included studies, 26 fulfilled at least six (75%) of the eight QUADAS criteria and were considered good-quality papers. We found that most of the studies used native excretory-secretory antigens and recombinant cathepsin in ELISA tests for serological diagnosis of fascioliasis in humans, cattle, and sheep. The meta-analysis revealed that all antigens demonstrated good accuracy. The best results in terms of sensitivity [0.931-2.5% confidence interval (CI) and 0.985-97.5% CI] and specificity (0.959-2.5% CI and 0.997-97.5% CI) were found in human ES. rCL-1, ES, and rSAP-2 antigens gave the best results for the serum diagnosis of human and animal fasciolosis.
可在动物和人类中引发问题。肝片吸虫病可通过间接酶联免疫吸附测定（ELISA）免疫诊断测试进行诊断。其血清学诊断基于这种蠕虫分泌的重组抗原。我们使用PubMed和谷歌学术数据库来回顾已发表的关于血清学检测中用于鉴定人类、牛和羊体内抗肝片吸虫抗体的“具有免疫原性潜力的抗原”的文献。纳入了采用常见参考标准进行诊断测试的研究，进行敏感性和/或特异性双变量荟萃分析。在对纳入的33项研究进行的质量和偏倚易感性分析中，26项研究至少满足八项QUADAS标准中的六项（75%），被视为高质量论文。我们发现，大多数研究在ELISA检测中使用天然排泄-分泌抗原和重组组织蛋白酶来对人类、牛和羊的肝片吸虫病进行血清学诊断。荟萃分析显示，所有抗原均表现出良好的准确性。在人类排泄-分泌产物（ES）中发现了敏感性[0.931 - 2.5%置信区间（CI）和0.985 - 97.5% CI]和特异性（0.959 - 2.5% CI和0.997 - 97.5% CI）方面的最佳结果。rCL - 1、ES和rSAP - 2抗原在人类和动物肝片吸虫病的血清诊断中取得了最佳结果。
