Laboratorio de Parasitología, Facultad de Zootecnia, Universiad Nacional Agraria La Molina, Lima, Peru.
Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
Am J Trop Med Hyg. 2019 Jul;101(1):59-61. doi: 10.4269/ajtmh.18-0988.
We compared the diagnostic performance of the standard method (Kato-Katz) with two recently developed methods (Mini-FLOTAC and Flukefinder) for the detection and quantification of eggs in human stool. Uninfected human stool samples were artificially spiked with eggs to reach final concentrations of 14, 28, 41, or 96 eggs per gram of stool (epg). Only Flukefinder showed 100% sensitivity in all but the samples with the lowest concentration of eggs (14 epg), in which it had a sensitivity of 60%. Each of the methods underestimated the true fecal egg counts (FECs), Flukefinder resulting in the most biased egg counts (egg counts 0.18 times lower than the expected FECs). Only the Flukefinder resulted in more precise results (coefficient of variance < 30%) from FECs of 96 epg onward. The outcome of this study indicates that the Flukefinder is a useful alternative diagnostic method for human fascioliasis in stool.
我们比较了标准方法（加藤厚法）和两种最近开发的方法（Mini-FLOTAC 和 Flukefinder）在检测和定量人粪便中虫卵的诊断性能。未感染的人粪便样本被人工添加虫卵，达到每克粪便中 14、28、41 或 96 个虫卵（epg）的最终浓度。只有 Flukefinder 在除了虫卵浓度最低的样本（14 epg）之外的所有样本中均显示出 100%的灵敏度，在这些样本中，它的灵敏度为 60%。所有方法均低估了真实的粪便虫卵计数（FEC），Flukefinder 导致的虫卵计数偏差最大（计数比预期的 FEC 低 0.18 倍）。只有 Flukefinder 从 FEC 为 96 epg 开始产生更精确的结果（变异系数 < 30%）。本研究的结果表明，Flukefinder 是一种用于粪便中人片形吸虫病的有用替代诊断方法。
