Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510640, People's Republic of China.
Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Characteristic Plant Extraction Laboratory, Yunnan Provincial Center for Research & Development of Natural Products, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Pharmacy and School of Chemical Science and Technology, Yunnan University, Kunming, 650091, People's Republic of China.
J Nat Prod. 2023 Oct 27;86(10):2348-2359. doi: 10.1021/acs.jnatprod.3c00543. Epub 2023 Sep 22.
Eleven diterpenoids, wulfenioidins D-N (-), classified into five distinct carbon skeletons with one unreported framework, and four modified abietane diterpenoids were isolated from the whole plant of . The structures and absolute configurations were characterized by spectroscopic methods, single-crystal X-ray diffraction, and electronic circular dichroism analyses. Compounds and exhibited activity against Zika virus (ZIKV) with EC values of 8.07 and 8.50 μM, respectively, and showed no significant cytotoxicity toward Vero cells at 100 μM. Western blot and immunofluorescence experiments showed that compounds and interfered with the replication of the ZIKV by inhibiting the expression of the ZIKV envelope (E) protein.
从 全株植物中分离得到 11 个二萜类化合物，包括 5 种不同碳骨架的 wulfenioidins D-N（-），其中 1 种为未见报道的骨架，以及 4 种修饰的松香烷二萜类化合物。通过光谱方法、单晶 X 射线衍射和电子圆二色分析确定了它们的结构和绝对构型。化合物 和 对 Zika 病毒（ZIKV）表现出活性，EC 值分别为 8.07 和 8.50 μM，在 100 μM 时对 Vero 细胞没有明显的细胞毒性。Western blot 和免疫荧光实验表明，化合物 和 通过抑制 ZIKV 包膜（E）蛋白的表达来干扰 ZIKV 的复制。
