Romano N, Russo Alesi D, La Licata R, Tolone G
Microbiologica. 1986 Oct;9(4):405-13.
The effects of various reaction conditions and the influence of urea and its hydrolysis products on Ureaplasma ATP synthesis were assayed using of a pH stat system which avoids the unfavourable changes of the external pH. The results proved that ATP synthesis required the presence of an optimal phosphate concentration and was dependent on the urea content of the reaction mixture. The data suggest that urea is the energetic source for ureaplasma growth and ammonium ions and pH could interfere in an additive way with the production of energy, only when the starting pH of the medium is kept in a slightly basic range.
使用pH计系统测定了各种反应条件的影响以及尿素及其水解产物对脲原体ATP合成的影响，该系统可避免外部pH值的不利变化。结果证明，ATP合成需要存在最佳磷酸盐浓度，并且依赖于反应混合物中的尿素含量。数据表明，尿素是脲原体生长的能量来源，并且只有当培养基的起始pH值保持在略碱性范围内时，铵离子和pH值才会以累加方式干扰能量的产生。
