Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, Spain.
Departamento de Bioquímica y Biología Molecular, Universitat de València, Dr Moliner 50, 46100 Burjassot, Spain.
Biosensors (Basel). 2022 Nov 7;12(11):980. doi: 10.3390/bios12110980.
Quality assurance and food safety are of great concern within the food industry because of unknown quantities of allergens often present in food. Therefore, there is an ongoing need to develop rapid, sensitive, and easy to use methods that serve as an alternative to mass spectrometry and enzyme-linked immunosorbent assay (ELISA) for monitoring food safety. Lateral flow immunoassay is one of the most used point-of-need devices for clinical, environmental, and food safety applications. Compared to traditional methods, it appears to be a simple and fast alternative for detecting food allergens. However, its reliability is frequently questioned due to the lack of quantitative information. In this study, a lateral flow microimmunoassay (LFµIA) is presented that integrates up to 36 spots in microarray format in a single strip, providing semi-quantitative information about the level of allergens, positive and negative controls, internal calibration, and hook effect. The LFµIA has been evaluated for the on-site simultaneous and reliable quantification of almond and peanut allergens as a proof of concept, demonstrating high sensitivity (185 and 229 µg/kg, respectively), selectivity (77%), and accuracy (RSD 5-25%) when analyzing commercial allergen-suspicious food consumables.
质量保证和食品安全是食品工业非常关注的问题，因为食品中经常存在未知数量的过敏原。因此，需要不断开发快速、敏感、易于使用的方法，作为替代质谱和酶联免疫吸附测定（ELISA）的方法来监测食品安全。侧向流动免疫分析是用于临床、环境和食品安全应用的最常用的即时检测设备之一。与传统方法相比，它似乎是一种简单快速的替代方法，用于检测食物过敏原。然而，由于缺乏定量信息，其可靠性经常受到质疑。在这项研究中，提出了一种侧向流动微免疫分析（LFµIA），它将多达 36 个斑点集成在微阵列格式的单个条带中，提供有关过敏原水平、阳性和阴性对照、内部校准和钩状效应的半定量信息。该 LFµIA 已被评估用于现场同时可靠地定量杏仁和花生过敏原，作为概念验证，在分析商业过敏原可疑食品消费品时，表现出高灵敏度（分别为 185 和 229 µg/kg）、选择性（77%）和准确性（RSD 5-25%）。
