Molecular Allergy Research Laboratory, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD, Australia.
Australian Institute of Tropical Health and Medicine, James Cook University, Townsville, QLD, Australia.
Methods Mol Biol. 2024;2717:351-365. doi: 10.1007/978-1-0716-3453-0_24.
Food allergens have been traditionally identified using biomolecular and immunological approaches. However, the techniques used in extracting proteins from the food source to be analyzed may hinder the availability of all proteins when assessing immunological allergenicity. Additionally, depending on the number and pool of patient sera used to detect the IgE antibody-binding allergens, some allergens may not be detected if not all the patients in the pool are sensitized to all the allergens. To overcome these limitations, we describe an additional approach before the in vitro approaches, by analyzing the transcriptome in silico for all putative allergens within the analyzed food source.
传统上，食物过敏原是通过生物分子和免疫学方法来鉴定的。然而，在从待分析的食物来源中提取蛋白质时所使用的技术，可能会在评估免疫变应原性时阻碍所有蛋白质的可用性。此外，根据用于检测 IgE 抗体结合变应原的患者血清数量和池，某些变应原可能不会被检测到，如果池中的并非所有患者都对所有变应原敏感的话。为了克服这些限制，我们在体外方法之前描述了一种额外的方法，通过对分析食物来源中的所有潜在变应原进行计算机分析来分析转录组。
