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鳗鱼电器官:钙调蛋白系统高表达

Eel electric organ: hyperexpressing calmodulin system.

作者信息

Munjaal R P, Connor C G, Turner R, Dedman J R

出版信息

Mol Cell Biol. 1986 Mar;6(3):950-4. doi: 10.1128/mcb.6.3.950-954.1986.

DOI:10.1128/mcb.6.3.950-954.1986
PMID:3773897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367596/
Abstract

The electroplax of the electric eel Electrophorus electricus is the most abundant source of the calcium-binding protein calmodulin. The electroplax has 250 times the amount of calmodulin and its mRNA than eel skeletal muscle. Our data suggest that there is no major difference in gene copies, the degree of methylation, or genome rearrangement of the calmodulin gene in DNAs from eel electroplax and muscle. Differences in the calmodulin-binding proteins in electroplax and muscle suggest a differential role for the functional expression of calmodulin in cellular regulation.

摘要

电鳗(Electrophorus electricus)的电板是钙结合蛋白钙调蛋白最丰富的来源。电板中的钙调蛋白及其mRNA含量是鳗鱼骨骼肌的250倍。我们的数据表明,电鳗电板和肌肉的DNA中,钙调蛋白基因的基因拷贝数、甲基化程度或基因组重排没有重大差异。电板和肌肉中钙调蛋白结合蛋白的差异表明,钙调蛋白在细胞调节中的功能表达具有不同的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/caf266df7111/molcellb00087-0216-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/92930d04f511/molcellb00087-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/a55b22cb3862/molcellb00087-0215-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/ea27c2f5e8a1/molcellb00087-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/caf266df7111/molcellb00087-0216-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/92930d04f511/molcellb00087-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/a55b22cb3862/molcellb00087-0215-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/ea27c2f5e8a1/molcellb00087-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/963d/367596/caf266df7111/molcellb00087-0216-b.jpg

相似文献

1
Eel electric organ: hyperexpressing calmodulin system.鳗鱼电器官:钙调蛋白系统高表达
Mol Cell Biol. 1986 Mar;6(3):950-4. doi: 10.1128/mcb.6.3.950-954.1986.
2
Calcium-binding proteins in electroplax and skeletal muscle. Comparison of the parvalbumin and phosphodiesterase activator protein of Electrophorus electricus.电鳐和骨骼肌中的钙结合蛋白。电鳐小白蛋白与磷酸二酯酶激活蛋白的比较。
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3
Isolation and purification of calcium-binding protein from electroplax of Electrophorus electricus.从电鳗电板中分离和纯化钙结合蛋白。
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Identification of multiple species of calmodulin messenger RNA using a full length complementary DNA.使用全长互补DNA鉴定钙调蛋白信使核糖核酸的多种物种。
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Isolation of the structural gene for calmodulin.钙调蛋白结构基因的分离。
Ann N Y Acad Sci. 1980;356:110-8. doi: 10.1111/j.1749-6632.1980.tb29604.x.
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Unique patterns of transcript and miRNA expression in the South American strong voltage electric eel (Electrophorus electricus).南美强电压电鳗(Electrophorus electricus)中转录本和微小RNA表达的独特模式。
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Purification of acetylcholine receptors from the muscle of Electrophorus electricus.从电鳗肌肉中纯化乙酰胆碱受体。
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Na+/K+-ATPase α-subunit (nkaα) isoforms and their mRNA expression levels, overall Nkaα protein abundance, and kinetic properties of Nka in the skeletal muscle and three electric organs of the electric eel, Electrophorus electricus.电鳗(Electrophorus electricus)骨骼肌和三个电器官中Na⁺/K⁺-ATP酶α亚基(nkaα)同工型及其mRNA表达水平、Nkaα蛋白总体丰度以及Nka的动力学特性。
PLoS One. 2015 Mar 20;10(3):e0118352. doi: 10.1371/journal.pone.0118352. eCollection 2015.
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A major second messenger mediator of Electrophorus electricus electric tissue is CaM kinase II.
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A cloned calmodulin structural gene probe is complementary to DNA sequences from diverse species.一个克隆的钙调蛋白结构基因探针与来自不同物种的DNA序列互补。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2330-4. doi: 10.1073/pnas.78.4.2330.

引用本文的文献

1
A tail of two voltages: Proteomic comparison of the three electric organs of the electric eel.两种电压的尾巴:电鳗三个电器官的蛋白质组比较。
Sci Adv. 2017 Jul 5;3(7):e1700523. doi: 10.1126/sciadv.1700523. eCollection 2017 Jul.

本文引用的文献

1
Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.细胞质斑点杂交。对多个小细胞或组织样本中相对mRNA水平的简单分析。
J Biol Chem. 1982 Aug 10;257(15):8569-72.
2
Positive correlation between calmodulin content and hepatoma growth rates.钙调蛋白含量与肝癌生长速率之间呈正相关。
Cancer Res. 1982 Jul;42(7):2571-4.
3
Calmodulin and the cell cycle: involvement in regulation of cell-cycle progression.钙调蛋白与细胞周期:参与细胞周期进程的调控
Cell. 1982 Jan;28(1):41-50. doi: 10.1016/0092-8674(82)90373-7.
4
Calmodulin--an intracellular calcium receptor.钙调蛋白——一种细胞内钙受体。
Nature. 1980 May 8;285(5760):73-7. doi: 10.1038/285073a0.
5
Localization of calmodulin in rat tissues.钙调蛋白在大鼠组织中的定位。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):366-70. doi: 10.1073/pnas.77.1.366.
6
A cloned calmodulin structural gene probe is complementary to DNA sequences from diverse species.一个克隆的钙调蛋白结构基因探针与来自不同物种的DNA序列互补。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2330-4. doi: 10.1073/pnas.78.4.2330.
7
Amplification of the metallothionein-I gene in cadmium-resistant mouse cells.金属硫蛋白-I基因在耐镉小鼠细胞中的扩增。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2110-4. doi: 10.1073/pnas.78.4.2110.
8
The role of calmodulin in human renal cell carcinoma.钙调蛋白在人类肾细胞癌中的作用。
Biochem Biophys Res Commun. 1983 Jul 29;114(2):843-9. doi: 10.1016/0006-291x(83)90858-6.
9
Increased content of calmodulin in human leukemia cells.人类白血病细胞中钙调蛋白含量增加。
Leuk Res. 1983;7(1):97-100. doi: 10.1016/0145-2126(83)90062-0.
10
Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster.黑腹果蝇卵子发生过程中绒毛膜蛋白基因的扩增
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1096-100. doi: 10.1073/pnas.77.2.1096.