Wang Yunyi, Li Gang, Kong Li, Lin Ling
State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin, China.
Anal Bioanal Chem. 2023 Nov;415(27):6733-6742. doi: 10.1007/s00216-023-04950-x. Epub 2023 Sep 23.
Non-invasive blood composition analysis based on dynamic spectrum (DS) theory has gained significant attention due to its non-invasive, simple, and fast performance. However, most of the multi-wavelength photoplethysmography (PPG) detection devices used to obtain DS are composed of halogen light sources and spectrometers and cannot detect effective PPG signals in the visible light short band (400-620 nm), which limits the detection accuracy of blood components with significant absorption spectral differences in that band. Therefore, this paper designs a multi-wavelength spectral acquisition system that can measure high signal-to-noise ratio (SNR > 65 dB) PGG signals at wavelengths of 405, 430, 450, 505, 520, and 570 nm and combines this system with a halogen lamp spectrometer acquisition system for non-invasive blood component detection. Furthermore, this paper collects the DS of 272 subjects with the combined system and establishes a predictive model for DS with the content of red blood cell (RBC) and hemoglobin (HGB) components. The results show that, compared with the halogen lamp spectrometer acquisition system, the correlation coefficient (Rp) of RBC and HGB prediction model established by the combined system has increased by 0.0619 and 0.0489, respectively, and the root mean square error (RMSE) has decreased by 0.08 1e12/L and 0.85 g/L, which confirm the feasibility of the designed multi-wavelength spectrum acquisition system to enhance the accuracy of blood component detection.
基于动态光谱（DS）理论的无创血液成分分析因其无创、操作简单且快速的特点而备受关注。然而，大多数用于获取DS的多波长光电容积脉搏波描记法（PPG）检测设备由卤素光源和光谱仪组成，无法检测可见光短波段（400 - 620nm）内有效的PPG信号，这限制了对该波段具有显著吸收光谱差异的血液成分的检测精度。因此，本文设计了一种多波长光谱采集系统，该系统能够在405、430、450、505、520和570nm波长下测量高信噪比（SNR > 65dB）的PPG信号，并将该系统与卤素灯光谱仪采集系统相结合用于无创血液成分检测。此外，本文利用该组合系统采集了272名受试者的DS，并建立了关于红细胞（RBC）和血红蛋白（HGB）成分含量的DS预测模型。结果表明，与卤素灯光谱仪采集系统相比，组合系统建立的RBC和HGB预测模型的相关系数（Rp）分别提高了0.0619和0.0489，均方根误差（RMSE）分别降低了0.08×10¹²/L和0.85g/L，这证实了所设计的多波长光谱采集系统提高血液成分检测精度的可行性。
