Mahatma Gandhi Vidyamandir's Karmaveer Bhausaheb Hiray Dental College & Hospital, Nashik, India.
Oral Oncol. 2023 Nov;146:106575. doi: 10.1016/j.oraloncology.2023.106575. Epub 2023 Sep 22.
Intravital microscopy (IVM) and optical coherency tomography (OCT) are powerful optical imaging tools that allow visualization of dynamic biological activities in living subjects with subcellular resolutions. They have been used in preclinical and clinical cancer imaging, providing insights into the complex physiological, cellular, and molecular behaviors of tumors. They have revolutionized cancer diagnosis and therapies, allowing for real-time observation of biologic processes in vivo, including angiogenesis and immune cell interactions. Recent developments in techniques for observing deep tissues of living animals have improved bioluminescent proteins, fluorescent proteins, fluorescent dyes, and detection technologies like two-photon excitation microscopy. These technologies have become indispensable tools in basic sciences, preclinical research, and modern drug development. In Vivo imaging can detect subcellular signaling or metabolic events in living animals, but depth-dependent signal attenuation limits the depth from which significant data can be obtained. Cancer cell motility and invasion are key features of metastatic tumors, but only a small portion of tumor cells are motile and metastasize due to genetic, epigenetic, and microenvironmental heterogeneities.
活体显微镜（IVM）和光学相干断层扫描（OCT）是强大的光学成像工具，可在活体内以亚细胞分辨率可视化动态生物活性。它们已被用于临床前和临床癌症成像，为肿瘤的复杂生理、细胞和分子行为提供了深入了解。它们彻底改变了癌症的诊断和治疗方法，使人们能够实时观察体内的生物学过程，包括血管生成和免疫细胞相互作用。观察活体动物深层组织的技术的最新发展提高了生物发光蛋白、荧光蛋白、荧光染料以及双光子激发显微镜等检测技术的性能。这些技术已经成为基础科学、临床前研究和现代药物开发中不可或缺的工具。在体成像可以检测活体动物中的亚细胞信号或代谢事件，但深度相关的信号衰减限制了可以获得有意义数据的深度。癌细胞的运动和侵袭是转移性肿瘤的关键特征，但由于遗传、表观遗传和微环境异质性，只有一小部分肿瘤细胞具有运动性和转移性。
