Hylden J L, Wilcox G L
Neuroscience. 1986 Oct;19(2):393-401. doi: 10.1016/0306-4522(86)90269-1.
The importance of the spinothalamic tract in pain transmission makes it an attractive candidate for study with respect to the effects of antinociceptive compounds. We have been interested in the analgesic actions of opioids and noradrenergic agents at the spinal level and have investigated the effects of these agents on extracellularly recorded nociceptive dorsal horn neurons in the rat. Spinothalamic tract cells were identified by antidromic activation from the somatosensory thalamus. Morphine was administered by bathing the spinal cord in an artificial cerebrospinal fluid solution which contained a known concentration of drug. We observed a dose-related inhibition, naloxone-reversible in some cases, of activity produced by spinally administered morphine in identified rat spinothalamic tract cells and dorsal horn nociceptive neurons. Morphine had no effect on stimulus-evoked responses of low threshold dorsal horn neurons.
脊髓丘脑束在疼痛传递中的重要性使其成为研究抗伤害感受性化合物作用的一个有吸引力的对象。我们一直对阿片类药物和去甲肾上腺素能药物在脊髓水平的镇痛作用感兴趣，并研究了这些药物对大鼠细胞外记录的伤害性背角神经元的影响。通过从体感丘脑进行逆向激活来识别脊髓丘脑束细胞。将含有已知浓度药物的人工脑脊液溶液灌注脊髓来给予吗啡。我们观察到，在已识别的大鼠脊髓丘脑束细胞和背角伤害性神经元中，脊髓给予吗啡所产生的活动受到剂量相关的抑制，在某些情况下这种抑制可被纳洛酮逆转。吗啡对低阈值背角神经元的刺激诱发反应没有影响。
