Gelgor Linda, Phillips Sally, Butkow Neil, Mitchell Duncan
Department of Physiology, University of the Witwatersrand Medical School, Parktown, Johannesburg 2193 South Africa.
Pain. 1986 Sep;26(3):353-359. doi: 10.1016/0304-3959(86)90063-1.
We have investigated the effects of mepyramine, an H1 receptor antagonist, and lysine acetylsalicylate, a cyclo-oxygenase inhibitor, on post-ischaemic hyperalgesia in rats. We induced tail ischaemia in conscious rats by applying a tourniquet until the rats exhibited coordinated escape behaviour, when we released the tourniquet. We assessed hyperalgesia, by measuring tail flick latency following tail immersion in water at 49 degrees C, immediately after releasing the tourniquet and then at 30 min intervals for 2 h. After pretreatment with the drug vehicles, tail flick latency decreased significantly following ischaemia. Pretreatment with mepyramine maleate (3 mg/kg), or lysine acetylsalicylate (400 mg/kg), injected subcutaneously, abolished the decrease. We conclude that both histamine release and prostanoid synthesis are involved in the post-ischaemic hyperalgesia.
我们研究了H1受体拮抗剂美吡拉敏和环氧化酶抑制剂赖氨酸阿司匹林对大鼠缺血后痛觉过敏的影响。我们通过应用止血带诱导清醒大鼠的尾部缺血，直到大鼠表现出协调的逃避行为时松开止血带。在松开止血带后立即以及随后每30分钟测量一次将尾巴浸入49摄氏度水中后的甩尾潜伏期，持续2小时，以此评估痛觉过敏。用药物载体进行预处理后，缺血后甩尾潜伏期显著缩短。皮下注射马来酸美吡拉敏（3mg/kg）或赖氨酸阿司匹林（400mg/kg）进行预处理可消除这种缩短。我们得出结论，组胺释放和前列腺素合成均参与缺血后痛觉过敏。
