Takeda Shiro, Ahhmed Abdulatef M, Sogawa Kazuki, Mouri Saki, Kaneko Sakurako, Sakata Ryoichi, Mizunoya Wataru
School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
Graduate School of Veterinary Science, Azabu University, Sagamihara, Kanagawa, Japan.
Anim Sci J. 2023 Jan-Dec;94(1):e13870. doi: 10.1111/asj.13870.
Venison, a type of game meat, has several health benefits because it contains not only high protein and low fat but also bioactive peptides with several physiological properties, including antioxidative and angiotensin-converting enzyme inhibitory properties. The aim of the present study was to investigate the antioxidant activity of venison treated by in vitro cooking and gastrointestinal digestion. We subjected venison along with pork and beef to in vitro cooking and digestion and assessed their antioxidant activity via 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH-RS) and hydrophilic oxygen radical absorbance capacity (H-ORAC) assays. The peptide contents of all types of cooked and digested meat samples were higher than those of the untreated and cooked samples. The DPPH-RS activities and H-ORAC of digested venison, pork, and beef were increased compared with those of untreated samples. DPPH-RS activity was significantly higher in the digested venison samples than in the digested pork and beef samples. In this study, several fractions of digested venison from the chromatography exhibited DPPH-RS activity. Peptide analysis, using liquid-chromatography with tandem mass spectrometry, unveiled two peptides DIDDLELTLAK and TQTVCNFTDGALVQHQEWDGK with high DPPH-RS activities. Thus, venison is a rich source of antioxidant peptides and potentially demonstrate an antioxidation ability by digestive enzymes in vivo.
鹿肉是一种野味，具有多种健康益处，因为它不仅含有高蛋白和低脂肪，还含有具有多种生理特性的生物活性肽，包括抗氧化和血管紧张素转换酶抑制特性。本研究的目的是调查体外烹饪和胃肠道消化处理后的鹿肉的抗氧化活性。我们将鹿肉与猪肉和牛肉进行体外烹饪和消化，并通过2,2-二苯基-1-苦基肼自由基清除（DPPH-RS）和亲水氧自由基吸收能力（H-ORAC）测定来评估它们的抗氧化活性。所有类型的熟肉和消化肉样品中的肽含量均高于未处理和熟肉样品。与未处理样品相比，消化后的鹿肉、猪肉和牛肉的DPPH-RS活性和H-ORAC有所增加。消化后的鹿肉样品中的DPPH-RS活性显著高于消化后的猪肉和牛肉样品。在本研究中，色谱法得到的消化鹿肉的几个组分表现出DPPH-RS活性。使用液相色谱-串联质谱进行的肽分析揭示了两种具有高DPPH-RS活性的肽DIDDLELTLAK和TQTVCNFTDGALVQHQEWDGK。因此，鹿肉是抗氧化肽的丰富来源，并可能在体内通过消化酶表现出抗氧化能力。
