Fertility Medical Group, Av. Brigadeiro Luis Antonio, 4545, São Paulo - SP, Brazil01401-002.
Sapientiae Institute, Centro de Estudos e Pesquisa em Reprodução Humana Assistida, Rua Vieira Maciel, 62, São Paulo - SP, Brazil04503-040.
Zygote. 2023 Dec;31(6):570-576. doi: 10.1017/S0967199423000370. Epub 2023 Sep 25.
Our objective was to study whether serum anti-Müllerian hormone (AMH) concentrations were associated with embryo morphokinetic events. This retrospective cohort study was performed in a private university-affiliated fertilization centre between March 2019 and December 2020 and included 902 oocytes cultured in a time-lapse imaging incubator, obtained from 114 intracytoplasmic sperm injection cycles performed. The relationship between AMH concentrations and morphokinetic events was investigated by considering the clustering of data (multiple embryos/patient). Evaluated kinetic markers were time to pronuclei appearance (tPNa) and fading (tPNf), time to two (t2), three (t3), four (t4), five (t5), six (t6), seven (t7), and eight cells (t8), (tSB) and time to the start of blastulation (tSB) and to blastulation (tB). Significant inverse relationships were observed between serum AMH concentrations and tPNf, t3, t4, t5, t6, t7, t8, and tB. The AMH was positively correlated with the KIDScore and implantation rate. Increased serum AMH concentrations correlated with faster embryo development. The clinical implications of this effect on embryo development warrant further investigation.
我们的目的是研究血清抗苗勒管激素（AMH）浓度是否与胚胎形态动力学事件相关。这项回顾性队列研究于 2019 年 3 月至 2020 年 12 月在一所私立大学附属的受精中心进行，共纳入了 114 个卵胞浆内单精子注射周期中的 902 个卵，这些卵在延时成像培养箱中培养。通过考虑数据聚类（多个胚胎/患者），研究了 AMH 浓度与形态动力学事件之间的关系。评估的动力学标志物包括原核出现时间（tPNa）和消失时间（tPNf）、二细胞时间（t2）、三细胞时间（t3）、四细胞时间（t4）、五细胞时间（t5）、六细胞时间（t6）、七细胞时间（t7）、八细胞时间（t8）、桑椹胚形成时间（tSB）和开始桑椹胚形成时间（tSB）和桑椹胚形成时间（tB）。血清 AMH 浓度与 tPNf、t3、t4、t5、t6、t7、t8 和 tB 呈显著负相关。AMH 与 KIDScore 和着床率呈正相关。血清 AMH 浓度增加与胚胎发育加快有关。这种对胚胎发育的影响的临床意义值得进一步研究。
