Si Keyi, Ma Bingxin, Bai Jian, Wu Li, He Hui, Jin Lei, Huang Bo
Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Heliyon. 2024 Nov 26;10(23):e40686. doi: 10.1016/j.heliyon.2024.e40686. eCollection 2024 Dec 15.
The change of morphokinetic pattern in aneuploid embryos will facilitate the non-invasive selection of euploid embryos. In this study, we investigated the impact of different chromosomal abnormalities on the morphokinetic patterns of embryonic development.
Our cohort includes 939 time-lapse preimplantation genetic testing cycles performed between January 2019 and July 2022 at a single academic fertility center, with a total of 2876 biopsied blastocysts. Intracytoplasmic sperm injection, blastocyst culture, trophectoderm biopsy, time-lapse monitoring, and next-generation sequencing were performed.
After adjusting for patient- and cycle-related factors, ix morphokinetic parameters (t5, P = 0.006; t8, P = 0.048; tSB, P < 0.001; tB,P < 0.001; t5-t2, P = 0.004; tB-tSB, P < 0.001) were significant in multilevel mixed-effects logistic regression model analysis for morphokinetic parameters to predict euploid or aneuploid embryos. None of the patient- or cycle-related factors systematically affected any morphokinetic parameter. Morphokinetic parameters of late cleavage and blastocyst stages in embryos with chromosome fragment deletion (t4 to t8, tB, t5-t2, tB-tSB, ECC2, ECC3, s2, P < 0.05) or duplication (t4, t5, tSB, tB, t5-t2, P < 0.05) were prolonged, and the morphokinetic parameters of the blastocyst stage in monosomic embryos (tSB, tB, tB-tSB, P < 0.01) were prolonged. Partial or complete chromosome 20 or 22 deletion can cause significant delays in multiple parameters of cleavage and blastocyst stages (from t4 to tB, P < 0.05).
Our study found that different chromosomal abnormalities have different effects on the morphokinetic parameters. Significant delays in morphokinetic parameters at different stages were found in fragment-mutated embryos and monosomic embryos. This can provide insights into the pre-implantation development pattern of aneuploid embryos and help non-invasive embryo selection.
非整倍体胚胎形态动力学模式的改变将有助于对整倍体胚胎进行非侵入性选择。在本研究中，我们调查了不同染色体异常对胚胎发育形态动力学模式的影响。
我们的队列包括2019年1月至2022年7月在单一学术性生育中心进行的939个延时植入前基因检测周期，共有2876个活检囊胚。进行了卵胞浆内单精子注射、囊胚培养、滋养外胚层活检、延时监测和下一代测序。
在对患者和周期相关因素进行调整后，在用于预测整倍体或非整倍体胚胎的形态动力学参数的多水平混合效应逻辑回归模型分析中，九个形态动力学参数（t5，P = 0.006；t8，P = 0.048；tSB，P < 0.001；tB，P < 0.001；t5 - t2，P = 0.004；tB - tSB，P < 0.001）具有显著性。没有患者或周期相关因素系统性地影响任何形态动力学参数。染色体片段缺失（t4至t8、tB、t5 - t2、tB - tSB、ECC2、ECC3、s2，P < 0.05）或重复（t4、t5、tSB、tB、t5 - t2，P < 0.05）的胚胎中，晚期卵裂和囊胚期的形态动力学参数延长，单体胚胎中囊胚期的形态动力学参数（tSB、tB、tB - tSB，P < 0.01）延长。20号或22号染色体部分或完全缺失可导致卵裂和囊胚期多个参数显著延迟（从t4到tB，P < 0.05）。
我们的研究发现不同染色体异常对形态动力学参数有不同影响。在片段突变胚胎和单体胚胎中发现不同阶段的形态动力学参数有显著延迟。这可为非整倍体胚胎的植入前发育模式提供见解，并有助于非侵入性胚胎选择。
