Department of Plant Pathology, Washington State University, Pullman, WA 99164, U.S.A.
Key Laboratory of Integrated Crop Pest Management of Anhui Province, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Plant Dis. 2024 Mar;108(3):624-634. doi: 10.1094/PDIS-07-23-1458-RE. Epub 2024 Mar 4.
is a necrotrophic fungal pathogen causing white mold on many important economic crops. Recently, some mycoviruses such as hypovirulence-associated DNA virus 1 (SsHADV-1) converted into a beneficial symbiont that helps plants manage pathogens and other stresses. To explore the potential use of SsHADV-1 as a biocontrol agent in the United States and to test the efficacy of SsHADV-1-infected United States isolates in managing white mold and other crop diseases, SsHADV-1 was transferred from the Chinese strain DT-8 to United States isolates of . SsHADV-1 is readily transmitted horizontally among United States isolates of and consistently conferred hypovirulence to its host strains. Biopriming of dry bean seeds with hypovirulent strains enhanced resistance to white mold, gray mold, and Rhizoctonia root rot. To investigate the underlying mechanisms, endophytic growth of hypovirulent in dry beans was confirmed using PCR, and the expression of 12 plant defense-related genes were monitored before and after infection. The results indicated that the endophytic growth of SsHADV-1-infected strains in plants stimulated the expression of plant immunity pathway genes that assisted a rapid response from the plant to fungal infection. Finally, application of the seed biopriming technology with SsHADV-1-infected hypervirulent strain has promise for the biological control of several diseases of wheat, pea, and sunflower.
是一种引起许多重要经济作物白霉病的坏死型真菌病原体。最近，一些真菌病毒，如弱毒相关 DNA 病毒 1（SsHADV-1），转化为有益的共生体，帮助植物管理病原体和其他压力。为了探索 SsHADV-1 在美国作为生物防治剂的潜在用途，并测试感染 SsHADV-1 的美国分离株在管理白霉病和其他作物病害方面的功效，将 SsHADV-1 从中国菌株 DT-8 转移到美国 分离株中。SsHADV-1 易于在 美国分离株之间水平传播，并一致赋予其宿主菌株弱毒特性。用弱毒 菌株对干豆种子进行生物引发处理可增强对白霉病、灰霉病和立枯丝核菌根腐病的抗性。为了研究潜在的机制，使用 PCR 确认了弱毒 在干豆中的内生生长，并且在感染前后监测了 12 个与植物防御相关的基因的表达。结果表明，感染 SsHADV-1 的菌株在植物中的内生生长刺激了植物免疫途径基因的表达，这些基因协助植物对真菌感染做出快速反应。最后，用感染了强毒的 SsHADV-1 的种子生物引发技术处理具有应用前景，可用于防治小麦、豌豆和向日葵的几种病害。
