Department of Obstetrics, The Second Hospital of Shandong University, Jinan, Shandong, P.R. China.
Department of Digestive Disease, The Second Hospital of Shandong University, Jinan, Shandong, P.R. China.
J Obstet Gynaecol. 2023 Dec;43(2):2259982. doi: 10.1080/01443615.2023.2259982. Epub 2023 Sep 24.
Preeclampsia (PE) is a serious pregnancy complication, resulting in potentially life-threatening conditions for both mother and foetus. It is worth noting that early-onset PE has become a great challenge for clinicians due to its complex manifestation, rapid progression and serious complications. This study aims to investigate differential serum proteome profiles in patients with early-onset PE.
Each serum sample was separated using a nanoliter flow rate Easy-nLC chromatography system. Then the samples were analysed by mass spectrometry. Bioinformatics analyses were conducted to analyse the functional categories or signal transduction pathways for differentially abundant proteins. Key proteins identified by mass spectrometry were verified by ELISA.
We found 30 and 34 proteins were upregulated and downregulated in early-onset PE patients ( = 3) vs controls ( = 3), respectively. Functional enrichment analysis revealed differentially expressed proteins related to the immune response and regulation of peptidase activity. ELISA confirmed that there were lower CSH1 levels and higher LPA concentrations in the serum samples of early-onset PE patients (n = 22) than in healthy controls ( = 19) ( < 0.05 for CSH1 and < 0.001 for LPA).
This study revealed the critical features of serum proteins in early-onset PE patients. LPA and CSH1 may serve as biomarkers for early-onset PE diagnosis and therapy.
子痫前期（PE）是一种严重的妊娠并发症，可导致母婴生命受到威胁。值得注意的是，由于其临床表现复杂、进展迅速且并发症严重，早发型 PE 已成为临床医生的巨大挑战。本研究旨在探讨早发型 PE 患者血清蛋白质组的差异表达谱。
使用纳升流速 Easy-nLC 色谱系统分离每个血清样本。然后通过质谱分析法对样品进行分析。通过生物信息学分析对差异丰度蛋白的功能类别或信号转导通路进行分析。通过 ELISA 验证质谱法鉴定的关键蛋白。
我们发现，30 种蛋白在早发型 PE 患者（n=3）血清中上调，34 种蛋白下调，而对照组（n=3）则没有。功能富集分析显示，差异表达蛋白与免疫反应和肽酶活性调节有关。ELISA 证实，早发型 PE 患者（n=22）血清中 CSH1 水平较低，LPA 浓度较高，而健康对照组（n=19）则相反（CSH1 的 < 0.05，LPA 的 < 0.001）。
本研究揭示了早发型 PE 患者血清蛋白的关键特征。LPA 和 CSH1 可能作为早发型 PE 诊断和治疗的生物标志物。
