Wang Qian, Song Juan, Zhang Jinlian, Qin Xiaojuan, Kang Yihao, Huang Shilv, Zhou Shengmao, Chen Tingsu
Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China.
Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China.
Front Microbiol. 2023 Sep 7;14:1203796. doi: 10.3389/fmicb.2023.1203796. eCollection 2023.
Ginger is one of the important spice crops in the world. Due to the prevalence of ginger wilt disease and the lack of effective prevention and control methods, the planting area, total production and value have declined sharply, which have become a key factor restricting ginger industry development in China. Understanding the influence of microbial agents on the rhizosphere microbiota of ginger will facilitate developing novel technologies for the prevention and control of ginger wilt disease. In the new planting and continuous cropping ginger fields, using large-root ginger and microbial agents, two inoculation levels (inoculation and no inoculation) were designed, and high-throughput sequencing technology was used to study the bacterial community structure in the rhizosphere soil at mature stage of ginger. The results showed that newly planted ginger showed a significant yield advantage over continuous cropping ginger, with a yield increase of 39% to 56%, and the lowest ginger wilt disease index. The community structure at the phylum level of soil bacteria in each treatment was very similar to that in the control, but the abundance of some taxonomic units changed significantly. The four dominant phyla of bacteria in mature ginger rhizosphere soil were , , , and , accounting for 72.91% to 89.09% of the total. The microbial agent treatment of continuous cropping had beneficial microorganisms such as and with abundances increased by 12.2% and 17.1%, respectively, compared to the control. The microbial inoculant treatment of newly planted ginger increased the abundance of and by 34.4% and 10.7%, respectively, compared to the control. The composition of bacterial communities were affected by changes in soil properties. Redundancy analysis showed that the hydrolysable nitrogen, available phosphorus, available potassium, and organic matter were significantly related to the composition of soil bacterial communities. Therefore, the microbial agents can not only promote the proliferation of beneficial microorganisms in the continuous cropping soil but also further reshape the soil bacterial community structure by changing the soil physicochemical properties such as effective phosphorus. These results provided a reference for related research on the impact of ginger continuous cropping on soil environment and soil management improvement in ginger fields.
生姜是世界上重要的香料作物之一。由于姜瘟病的普遍发生以及缺乏有效的防治方法，生姜的种植面积、总产量和产值急剧下降，已成为制约我国生姜产业发展的关键因素。了解微生物制剂对生姜根际微生物群的影响将有助于开发防治姜瘟病的新技术。在新种植和连作姜田中，以大肉姜和微生物制剂为材料，设计了两个接种水平（接种和不接种），并采用高通量测序技术研究生姜成熟期根际土壤细菌群落结构。结果表明，新种植生姜比连作生姜具有显著的产量优势，增产39%至56%，且姜瘟病病情指数最低。各处理土壤细菌门水平的群落结构与对照非常相似，但部分分类单元的丰度发生了显著变化。生姜成熟根际土壤中细菌的四个优势门为 、 、 、 ，占总数的72.91%至89.09%。连作土壤微生物制剂处理后，有益微生物如 和 的丰度分别比对照增加了12.2%和17.1%。新种植生姜微生物菌剂处理后， 和 的丰度分别比对照增加了34.4%和10.7%。细菌群落组成受土壤性质变化的影响。冗余分析表明，水解氮、有效磷、速效钾和有机质与土壤细菌群落组成显著相关。因此，微生物制剂不仅能促进连作土壤中有益微生物的增殖，还能通过改变有效磷等土壤理化性质进一步重塑土壤细菌群落结构。这些结果为生姜连作对土壤环境影响及姜田土壤管理改良的相关研究提供了参考。
