Dhende Suhas, Pathuthara Saleem, Prabhudesai Neelam, Shinde Dipak, Karnik Nupur, Deodhar Kedar
Department of Cytopathology, Tata Memorial Hospital, Tata Memorial Centre, Homi Bhabha National Institute, Mumbai, Maharashtra, India.
Department of Surgical Pathology, Tata Memorial Hospital, Tata Memorial Centre, Homi Bhabha National Institute, Mumbai, Maharashtra, India.
J Cytol. 2023 Jul-Sep;40(3):126-132. doi: 10.4103/joc.joc_34_23. Epub 2023 Aug 14.
Cellblock (CB) with immunohistochemistry (IHC) is practically indispensable in the diagnostic workup of serous effusions; however, CB requires a minimum of 15-20 h for routine histopathological processing. A reduction in processing time can expedite a faster diagnosis.
This study was undertaken to evaluate the utility of the heat-induced CB (HICB) technique.
Two sets of agar-embedded CBs were processed from 50 effusion samples. CBs were further processed by conventional and rapid methods. Conventional CBs (CCB) were processed in a histoprocessor, whereas rapid CB was processed in a heated water bath with an agitation facility. For HICB processing, dehydration and clearing were performed at 50°C followed by paraffin wax impregnation at 65°C temperature. From both CBs, sections of 5 um thickness were cut and stained with hematoxylin and eosin (H and E). Cell morphology, cost, and time were compared between the two methods. The feasibility of IHC was attempted in a few cases.
HICB was completed within 4.30 h compared with CCB. Diagnoses on both CBs were concordant in all the cases. Incomplete dehydration was noted in six (12%) cases, but the diagnosis was not compromised. No additional cost was involved in HICB. On IHC, both HICB and CCB exhibited equivalent expression.
HICB is a rapid, innovative, simple, and cost-effective technique and expedites faster diagnosis. It does not require any advanced equipment.
细胞块（CB）联合免疫组织化学（IHC）在浆液性积液的诊断检查中几乎不可或缺；然而，CB的常规组织病理学处理至少需要15 - 20小时。缩短处理时间可以加快诊断速度。
本研究旨在评估热诱导细胞块（HICB）技术的实用性。
从50份积液样本中制备两组琼脂包埋的细胞块。细胞块进一步采用传统方法和快速方法进行处理。传统细胞块（CCB）在组织处理仪中处理，而快速细胞块在带有搅拌装置的热水浴中处理。对于HICB处理，在50°C进行脱水和透明，然后在65°C进行石蜡包埋。从两种细胞块上切取5μm厚的切片，并用苏木精和伊红（H&E）染色。比较两种方法的细胞形态、成本和时间。在少数病例中尝试了免疫组化的可行性。
与CCB相比，HICB在4.30小时内完成。所有病例中两种细胞块的诊断结果一致。6例（12%）出现脱水不完全的情况，但诊断不受影响。HICB没有额外成本。在免疫组化方面，HICB和CCB表现出同等的表达。
HICB是一种快速、创新、简单且具有成本效益的技术，可加快诊断速度。它不需要任何先进设备。
