Weerasuriya Nimalka M, Kukolj Katarina, Spencer Rebecca, Sveshnikov Dmitry, Thorn R Greg
Department of Biology, University of Western Ontario, London, ON, Canada.
School of Science and the Environment, Grenfell Campus, Memorial University, Corner Brook, NL, Canada.
Front Fungal Biol. 2022 Mar 1;3:805127. doi: 10.3389/ffunb.2022.805127. eCollection 2022.
Showy lady's slipper ( Walter, Orchidaceae) and black ash ( Marshall, Oleaceae) often co-occur in close proximity in fens in western Newfoundland, Canada. Metabarcoding of DNA extracted from root samples of both species following surface sterilization, and others without surface sterilization was used to determine if there were shared fungal endophytes in the roots of both species that could form a common mycorrhizal network between them. A wide variety of fungi were recovered from primers amplifying the nuclear ribosomal internal transcribed spacer region (ITS2). Sixty-six fungal sequences were shared by surface-sterilized roots of both orchid and ash, among them arbuscular mycorrhizal fungi ( and ), ectomycorrhizal fungi ( and ), the broad-host root endophyte , along with root pathogens (, and ) and fungi of unknown function. There appear to be multiple fungi that could form a common mycorrhizal network between and , which might explain their frequent co-occurrence. Transfer of nutrients or carbon between the orchid and ash via one or more of the shared fungal endophytes remains to be demonstrated.
艳丽的拖鞋兰（沃尔特，兰科）和黑梣（马歇尔，木犀科）在加拿大纽芬兰西部的沼泽中常常紧密相邻生长。对两种植物经表面消毒和未经表面消毒的根样本提取的DNA进行代谢组学分析，以确定两种植物的根中是否存在可在它们之间形成共同菌根网络的共享真菌内生菌。从扩增核糖体内部转录间隔区2（ITS2）的引物中回收了多种真菌。兰花和梣经表面消毒的根共有66个真菌序列，其中包括丛枝菌根真菌（和）、外生菌根真菌（和）、广宿主根内生菌，以及根病原体（、和）和功能未知的真菌。似乎有多种真菌可以在和之间形成共同的菌根网络，这可能解释了它们频繁共生的现象。兰花和梣之间通过一种或多种共享真菌内生菌进行养分或碳的转移仍有待证明。
