Prevalence of , and in symptomatic and asymptomatic patients.

The purpose of the present study was to assess the prevalence of (), () and () in a Romanian population considering the presence or absence of genital symptoms. Urethral and vaginal samples were collected from patients presenting at 'Ponderas' Academic Hospital (Bucharest, Romania) from January 2021 to December 2021. A total of 266 samples were obtained from two groups of patients: Symptomatic subjects with urethritis, prostatitis, vaginitis or both urethritis and prostatitis (n=59; 22%), and asymptomatic subjects (n=207; 78%). and kits were used to assess the presence of and , and , respectively. The symptomatic subjects comprised 27 patients with urethritis symptoms, of whom 4 (15%) were infected with and 1 (4%) was infected with . In addition, 23 (9%) of the patients had prostatitis-like symptoms, which in 3 (13%) of the patients was associated with and in 1 patient (4%) was associated with . None of the symptomatic patients were infected with . By contrast, 29 (14%) of the asymptomatic patients were discovered to be infected with , 13 (6%) were coinfected with both Mollicutes and 4 (2%) were infected with ; only 1 patient was positive for alone. Two patients (14%) who presented with and coinfection were also infected with . No patient with or alone was also positive for . Therefore, the most frequently identified pathogen populating the genital tract in both males and females was , followed by coinfection with and , and . As these infections are asymptomatic in numerous cases, this suggests that a thorough screening should be mandatory.