Department of Dermatology, 'Iuliu Haţieganu' University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Cell and Molecular Biology, 'Iuliu Haţieganu' University of Medicine and Pharmacy, Cluj-Napoca, Romania.
J Infect Dev Ctries. 2020 Apr 30;14(4):341-348. doi: 10.3855/jidc.11989.
Most sexually transmitted infections (STIs) are curable, but inappropriate treatment can lead to serious complications. The importance of setting up STI screening programs has been highlighted in various studies, the absence of such national programs accounting for the lack of STI statistics in Romania. The purpose of our study was to evaluate multiplex PCR as a screening method for the most common 6 STIs and establish their frequency in a group of symptomatic and asymptomatic patients. We aimed to highlight STI associations and correlations between STI pathogens and symptomatology, demographic status, antecedents or sexual partners.
A total of 249 patients, both symptomatic and asymptomatic, were included in this study. Chlamydia trachomatis (CT), Neisseia gonorrhoeae (NG), Trichomonas vaginalis (TV), Ureaplasma urealyticum (UU), Mycoplasma hominis (MH) and Mycoplasma genitalium (MG) were all identified in urine samples via multiplex Polymerase Chain Reaction (PCR). The SPSS IBM program was employed for statistical analysis.
32.12% of the patients were found positive, some presenting multiple infections. The results are representative for the Romanian male population. 107 STI pathogens were identified, most frequent being CT, UU and NG. Several statistical correlations between patient characteristics and the presence of STIs have been demonstrated.
The results suggest that multiplex PCR meets all the prerequisites for a screening method, allowing the use of multiple specimens and enabling simultaneous detection of multiple pathogens in a short period of time. STI identification via multiplex PCR proved to be an effective method for quantifying their frequency in Romania.
大多数性传播感染（STI）是可治愈的，但不当的治疗可能会导致严重的并发症。在多项研究中强调了建立 STI 筛查计划的重要性，罗马尼亚缺乏这样的国家计划导致 STI 统计数据的缺失。我们的研究目的是评估多重 PCR 作为一种筛查最常见的 6 种 STI 的方法，并确定其在一组有症状和无症状患者中的频率。我们旨在强调 STI 的相关性以及 STI 病原体与症状、人口统计学状况、既往史或性伴侣之间的相关性。
本研究共纳入 249 例有症状和无症状的患者。通过多重聚合酶链反应（PCR）在尿液样本中检测沙眼衣原体（CT）、淋病奈瑟菌（NG）、阴道毛滴虫（TV）、解脲脲原体（UU）、人型支原体（MH）和生殖支原体（MG）。使用 SPSS IBM 程序进行统计分析。
32.12%的患者检测结果为阳性，部分患者存在多种感染。结果代表了罗马尼亚男性人群的情况。共鉴定出 107 种 STI 病原体，最常见的是 CT、UU 和 NG。还证明了患者特征与 STI 存在之间存在一些统计学相关性。
结果表明，多重 PCR 满足筛查方法的所有前提条件，允许使用多种标本，并能够在短时间内同时检测多种病原体。通过多重 PCR 鉴定 STI 被证明是一种有效方法，可以定量确定其在罗马尼亚的频率。
