Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun, Jilin 130024, China.
Department of Biology, School of Life Sciences, Institute of Plant and Food Science, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.
Proc Natl Acad Sci U S A. 2023 Oct 3;120(40):e2310881120. doi: 10.1073/pnas.2310881120. Epub 2023 Sep 25.
Cytonuclear disruption may accompany allopolyploid evolution as a consequence of the merger of different nuclear genomes in a cellular environment having only one set of progenitor organellar genomes. One path to reconcile potential cytonuclear mismatch is biased expression for maternal gene duplicates (homoeologs) encoding proteins that target to plastids and/or mitochondria. Assessment of this transcriptional form of cytonuclear coevolution at the level of individual cells or cell types remains unexplored. Using single-cell (sc-) and single-nucleus (sn-) RNAseq data from eight tissues in three allopolyploid species, we characterized cell type-specific variations of cytonuclear coevolutionary homoeologous expression and demonstrated the temporal dynamics of expression patterns across development stages during cotton fiber development. Our results provide unique insights into transcriptional cytonuclear coevolution in plant allopolyploids at the single-cell level.
细胞质 - 核基因组的不协调性可能伴随着异源多倍体的进化，这是由于不同的核基因组在一个只有一套前体细胞器基因组的细胞环境中融合的结果。一种协调潜在细胞质 - 核基因组不匹配的途径是偏向表达母本基因的重复（同系物），这些基因编码靶向质体和/或线粒体的蛋白质。在单个细胞或细胞类型水平上评估这种细胞质 - 核协同进化的转录形式仍然没有得到探索。我们使用来自三个异源多倍体物种的八个组织的单细胞（sc-）和单细胞核（sn-）RNAseq 数据，描述了细胞质 - 核协同进化的同系物表达在细胞类型特异性上的变化，并展示了在棉花纤维发育过程中不同发育阶段的表达模式的时间动态。我们的研究结果为在单细胞水平上研究植物异源多倍体中的转录细胞质 - 核协同进化提供了独特的见解。
