Ozdemir Merve, Ozdil Berrin, Abdikan Cemile Sinem Asker, Erisik Derya, Yesin Taha Kadir, Avci Cıgır Biray, Kurkutçu Yesim, Guler Gunnur, Aktug Huseyin
Department of Histology and Embryology, Faculty of Medicine, Ege University, Izmir 35100, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Ege University, Izmir 35100, Turkey; Department of Histology and Embryology, Faculty of Medicine, Suleyman Demirel University, Isparta 32260, Turkey.
Pathol Res Pract. 2023 Oct;250:154829. doi: 10.1016/j.prp.2023.154829. Epub 2023 Sep 22.
Melanoma is an aggressive tumor with a poor prognosis that worsens in the metastatic phase. Distruptions of epigenetic mechanisms is known to effect cancer stem cells (CSCs) activity. Malignant melanoma (MM) progression may be promoted by changes in the genetic structure of CSC. Thus, treatments that target epigenetic modifications could be a promising weapon, especially in melanoma. Here, we compared p300, HDAC9, and F-actin proteins in melanoma CSCs (CD133+), non-CSCs (CD133-) and CHL-1 cell line, as well as cell migration and division rates. At 4 and 6 h, P300 protein levels in CHL-1 and CD133 + were remarkably similar, and the CD133- showed increases in expression levels as the incubation period lengthened. HDAC9 protein intensity decreased in CHL-1, increased in the CD133-, and remained relatively unchanged in the CD133+ as the incubation period lengthened. The mean value of F-actin expression level increased in all cell group with time, when the highest increase observed in CHL-1. In conclusion, our studies contribute to the management of metastatic diseases in the future and offer new insight into the molecular basis of the initiation and progression of MM.
黑色素瘤是一种侵袭性肿瘤，预后较差，在转移阶段会恶化。已知表观遗传机制的破坏会影响癌症干细胞（CSCs）的活性。恶性黑色素瘤（MM）的进展可能由CSC基因结构的变化所促进。因此，针对表观遗传修饰的治疗可能是一种有前景的武器，尤其是在黑色素瘤治疗中。在这里，我们比较了黑色素瘤CSCs（CD133+）、非CSCs（CD133-）和CHL-1细胞系中p300、HDAC9和F-肌动蛋白蛋白，以及细胞迁移和分裂率。在4小时和6小时时，CHL-1和CD133+中的P300蛋白水平非常相似，而CD133-随着孵育时间的延长表达水平增加。随着孵育时间的延长，CHL-1中HDAC9蛋白强度降低，CD133-中增加，而CD133+中相对保持不变。随着时间的推移，所有细胞组中F-肌动蛋白表达水平的平均值均增加，其中CHL-1中增加最为明显。总之，我们的研究有助于未来转移性疾病的管理，并为MM的发生和进展的分子基础提供了新的见解。
