Emergency and Intensive Care Unit Center, Department of Intensive Care Unit, Chun'an First People's Hospital, Zhejiang Provincial People's Hospital Chun'an Branch, Hangzhou Medical College Affiliated Chun'an Hospital, Hangzhou, Zhejiang, People's Republic of China.
Clinical Laboratory of Chun'an First People's Hospital, Zhejiang Provincial People's Hospital Chun'an Branch, Hangzhou Medical College Affiliated Chun'an Hospital, Hangzhou, Zhejiang, People's Republic of China.
Anal Sci. 2024 Jan;40(1):37-45. doi: 10.1007/s44211-023-00427-0. Epub 2023 Sep 25.
A cholesterol biosensor was constructed by bimetallic (Au and Pt) and poly(amidoamine)-zeolite imidazole framework (PAMAM-ZIF-67). First, PAMAM-ZIF-67 nanomaterial was immobilized onto the electrode, and then Au and Pt were modified on the electrode by the electro-deposition method. Subsequently, cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) were fixed on the electrode. The stepwise modification procedures were recorded by impedance spectroscopy and voltammetry. The current response presented a linear relation to the logarithm of cholesterol content when content ranged between 0.00015 and 10.24 mM, and the minimum detection concentration reached 3 nM. The electrode was also used for the cholesterol assay in serum, which hinted at its potentially valuable in clinical diagnostics. An electrochemical biosensor based on gold nanoparticles, platinum nanoparticles, and polyamide-zeolitic imidazolate frameworks was developed for detection of cholesterol. First, polyamide-zeolitic imidazolate frameworks nanomaterial was fixed onto the electrode modified of mercaptopropionic acid by Au-S bond. Then, gold nanoparticles and platinum nanoparticles were electrodeposited on the above electrode. Subsequently, cholesterol oxidase and cholesterol esterase were co-immobilized on the surface of the modified electrode to fabricate the cholesterol biosensor. The biosensor has also been used for the measurement of cholesterol in human serum, which implied potential applications in biotechnology and clinical diagnostics.
构建了一种基于双金属（金和铂）和聚（酰胺-胺）-沸石咪唑酯骨架（PAMAM-ZIF-67）的胆固醇生物传感器。首先，将 PAMAM-ZIF-67 纳米材料固定在电极上，然后通过电沉积法在电极上修饰金和铂。随后，将胆固醇氧化酶（ChOx）和胆固醇酯酶（ChEt）固定在电极上。通过阻抗谱和伏安法记录逐步修饰过程。当胆固醇含量在 0.00015 至 10.24 mM 之间时，电流响应与胆固醇含量的对数呈线性关系，最小检测浓度达到 3 nM。该电极还用于血清中的胆固醇测定，这暗示了其在临床诊断中的潜在应用价值。一种基于金纳米粒子、铂纳米粒子和聚酰胺-沸石咪唑酯骨架的电化学生物传感器被开发用于检测胆固醇。首先，通过 Au-S 键将聚酰胺-沸石咪唑酯骨架纳米材料固定在修饰有巯基丙酸的电极上。然后，在上述电极上电沉积金纳米粒子和铂纳米粒子。随后，将胆固醇氧化酶和胆固醇酯酶共同固定在修饰电极的表面上，制备胆固醇生物传感器。该生物传感器还用于人血清中胆固醇的测定，这暗示了其在生物技术和临床诊断中的潜在应用。
