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基于 Au NPs/rGO-PAMAM-Fc 纳米材料的高灵敏度生物传感器用于胆固醇检测。

A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol.

机构信息

Department of Clinical Laboratory, Chun'an First People's Hospital (Zhejiang Provincial People's Hospital Chun'an Branch), Hangzhou, Zhejiang, China,

Department of Blood Transfusion, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China,

出版信息

Int J Nanomedicine. 2019 Jan 25;14:835-849. doi: 10.2147/IJN.S184013. eCollection 2019.

DOI:10.2147/IJN.S184013
PMID:30774337
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6354697/
Abstract

OBJECTIVE

This study aimed to construct a biosensor using Au nanoparticles (Au NPs) and reduced graphene-polyamide-amine-ferrocene (rGO-PAMAM-Fc) nanomaterials designed for rapid and sensitive detection of cholesterol.

MATERIALS AND METHODS

In this study, a highly sensitive biosensor based on Au NPs/ rGO-PAMAM-Fc nanomaterials was manufactured for detection of cholesterol. The rGO-PAMAM-Fc and Au NPs were modified on the surface of the electrode and then coated with cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) to develop the ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor.

RESULTS

The capability of rGO-PAMAM-Fc nanomaterials in fabricating a more efficient biosensor was validated through stability, selectivity and reproducibility checks. Under optimal conditions, the newly developed biosensor showed a linear relationship with logarithm of cholesterol concentration from 0.0004 to 15.36 mM ( =0.9986), and a low detection limit of 2 nM was obtained at the signal/noise ratio of 3.

CONCLUSION

The ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor was successfully applied for the measurement of cholesterol in human serum, which implies that the biosensor has a potential application in clinical diagnostics.

摘要

目的

本研究旨在构建一种使用金纳米粒子（Au NPs）和还原氧化石墨烯-聚酰胺-胺-二茂铁（rGO-PAMAM-Fc）纳米材料设计的生物传感器，用于快速灵敏地检测胆固醇。

材料和方法

在本研究中，制造了一种基于 Au NPs/rGO-PAMAM-Fc 纳米材料的高灵敏度生物传感器，用于检测胆固醇。rGO-PAMAM-Fc 和 Au NPs 被修饰在电极表面上，然后涂覆胆固醇氧化酶（ChOx）和胆固醇酯酶（ChEt），以开发 ChOx&ChEt/Au NPs/rGO-PAMAM-Fc 生物传感器。

结果

通过稳定性、选择性和重现性检查验证了 rGO-PAMAM-Fc 纳米材料在制造更有效生物传感器方面的能力。在最佳条件下，新开发的生物传感器显示出与胆固醇浓度对数从 0.0004 到 15.36 mM（=0.9986）之间的线性关系，并且在信噪比为 3 时获得了 2 nM 的低检测限。

结论

成功地将 ChOx&ChEt/Au NPs/rGO-PAMAM-Fc 生物传感器应用于人血清中胆固醇的测量，这表明该生物传感器在临床诊断中有潜在的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d89/6354697/8068fca1b7b8/ijn-14-835Fig8.jpg
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