School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Westville, South Africa, 4000.
Exp Clin Endocrinol Diabetes. 2023 Nov;131(11):569-576. doi: 10.1055/a-2181-6664. Epub 2023 Sep 26.
Chronic consumption of a high-calorie diet compromises the gut microbiota and the integrity of the intestinal wall, which causes translocation of bacterial lipopolysaccharides (LPS) into the blood. This elicits the secretion of pro-inflammatory cytokines, resulting in inflammation. However, how a high-fat high carbohydrate diet affects intestinal permeability and its possible role in the development of prediabetes have not been investigated. This study investigated the effects of HFHC diet-induced prediabetes on gut microbiota and intestinal permeability in male Sprague Dawley rats.
The animals were randomly assigned into the non-prediabetic (NPD) and diet-induced prediabetic (PD) groups (n=6) for 20 weeks. Then, the fecal samples were analyzed to measure the gut microbiota level of and in both animal groups. Blood glucose, plasma insulin, serum zonulin, plasma LPS, soluble CD14, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP), and intestinal fatty-acid binding protein (IFABP) concentrations were measured.
The PD group had a reduction in the and an increase in and levels compared to those in the NPD group. Blood glucose, insulin concentration, serum zonulin, and plasma sCD14 concentrations in the PD group increased significantly, while plasma LPS concentrations were similar to the NPD group. Concentrations of plasma TNF-α, IL-6, CRP, and IFABP, an intracellular protein expressed in the intestine, increased in PD compared to the NPD group.
the study results cumulatively suggest that chronic consumption of the HFHC diet may be associated with the dysregulation of gut microbiota, leading to increased intestinal permeability.
长期摄入高热量饮食会损害肠道微生物群和肠道壁的完整性，导致细菌脂多糖（LPS）易位进入血液。这会引发促炎细胞因子的分泌，导致炎症。然而，高脂肪高碳水化合物饮食如何影响肠道通透性及其在糖尿病前期发展中的可能作用尚未得到研究。本研究旨在探讨 HFHC 饮食诱导的糖尿病前期对雄性 Sprague Dawley 大鼠肠道微生物群和肠道通透性的影响。
动物被随机分为非糖尿病前期（NPD）和饮食诱导的糖尿病前期（PD）组（n=6），进行 20 周。然后分析粪便样本，以测量两组动物的肠道微生物群中 和 的水平。测量血糖、血浆胰岛素、血清紧密连接蛋白、血浆 LPS、可溶性 CD14、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、C 反应蛋白（CRP）和肠脂肪酸结合蛋白（IFABP）的浓度。
与 NPD 组相比，PD 组的 减少， 和 增加。PD 组的血糖、胰岛素浓度、血清紧密连接蛋白和血浆 sCD14 浓度显著升高，而血浆 LPS 浓度与 NPD 组相似。与 NPD 组相比，PD 组的血浆 TNF-α、IL-6、CRP 和 IFABP 浓度（一种在肠道中表达的细胞内蛋白）增加。
研究结果表明，长期摄入 HFHC 饮食可能与肠道微生物群失调有关，导致肠道通透性增加。
