Delevsky Yuriy Pavlovich, Zinchenko Olexandr Anatoliyovich
Sytenko Institute of Spine and Joint Pathology, National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine.
Ukrainian Scientific Pharmacopeial Center for the Quality of Medicines, Kharkiv, Ukraine.
Korean J Transplant. 2023 Sep 30;37(3):189-196. doi: 10.4285/kjt.23.0033.
Studies on the specificity of ABH antigen-antibody interactions at different pH values are rare. Therefore, the aim of this study was to estimate the effect of acidification of the reacting medium on the agglutinating ability of anti-A monoclonal antibodies (mABs) and their inhibition by glycoconjugates of red blood cell membranes.
Anti-A mABs were obtained from the Fourth International Workshop on Monoclonal Antibodies Against Human Red Blood Cell and Related Antigens (on July 19-20, 2002, in Paris, France). The glycoconjugates were isolated from erythrocytes' membranes. The inhibition of the lipid and protein isotypes of the blood group A antigen was assessed.
The acidic medium decreased the agglutinating ability of acid immunoglobulin M (IgM) anti-A mABs, in contrast to alkaline immunoglobulin G (IgG) mABs. Meanwhile, at pH 6.5, IgM antibodies exhibited a high adsorption capacity, while IgG antibodies demonstrated a strong adsorption capacity at an alkaline pH. mABs 2-19, 2-28, 2-22, and 2-8 were inhibited by the acidic lipid and protein glycotopes of erythrocyte membranes. However, mAB 2-8 was inhibited by both acidic and alkaline glycotope variants.
The agglutinating and adsorbing abilities of mABs, which are revealed at opposing pH values, should be taken into account during studies of antigen-antibody interactions.
关于不同pH值下ABH抗原-抗体相互作用特异性的研究很少。因此，本研究的目的是评估反应介质酸化对抗A单克隆抗体（mABs）凝集能力及其被红细胞膜糖缀合物抑制的影响。
抗A mABs取自第四届抗人红细胞及相关抗原单克隆抗体国际研讨会（2002年7月19 - 20日，法国巴黎）。糖缀合物从红细胞膜中分离出来。评估了血型A抗原的脂质和蛋白质同种型的抑制情况。
与碱性免疫球蛋白G（IgG）mABs相比，酸性介质降低了酸性免疫球蛋白M（IgM）抗A mABs的凝集能力。同时，在pH 6.5时，IgM抗体表现出高吸附能力，而IgG抗体在碱性pH下表现出强吸附能力。mABs 2 - 19、2 - 28、2 - 22和2 - 8被红细胞膜的酸性脂质和蛋白质糖位抑制。然而，mAB 2 - 8被酸性和碱性糖位变体均抑制。
在研究抗原-抗体相互作用时，应考虑在相反pH值下揭示的mABs的凝集和吸附能力。
