Børkje B, Skagen D W, Andersen K J, Schrumpf E
Scand J Gastroenterol. 1986 Oct;21(8):919-27. doi: 10.3109/00365528608996396.
The distribution of a series of mucosal enzymes along the large bowel was studied by analysis of homogenized biopsy specimens from five different segments, obtained from 20 control patients. The activities varied significantly between the segments for the membrane enzymes lactase (p less than 0.005), alkaline phosphatase (p less than 0.0005), leucyl-beta-naphthylamidase (p less than 0.0001), and 5'-nucleotidase (p less than 0.001) and the mitochondrial enzyme monoamine oxidase (p less than 0.0005) when tested by analysis of variance modified for repeated measurements. When paired comparisons between segments were evaluated, the enzyme activities of the proximal large bowel were significantly higher than those of distal segments. The levels of sucrase, neutral-alpha-glucosidase, gamma-glutamyltransferase, and lysosomal enzymes remained unchanged throughout the large intestine, as did the protein to DNA ratio. The results are compatible with the theory that different segments of the large bowel have different functions.
通过对来自20名对照患者的五个不同节段的活检标本进行匀浆分析，研究了一系列黏膜酶在大肠中的分布情况。对重复测量进行修正的方差分析显示，膜酶乳糖酶（p<0.005）、碱性磷酸酶（p<0.0005）、亮氨酰-β-萘酰胺酶（p<0.0001）和5'-核苷酸酶（p<0.001）以及线粒体酶单胺氧化酶（p<0.0005）在各节段之间的活性差异显著。当对各节段进行配对比较时，近端大肠的酶活性显著高于远端节段。整个大肠中蔗糖酶、中性α-葡萄糖苷酶、γ-谷氨酰转移酶和溶酶体酶的水平以及蛋白质与DNA的比值均保持不变。这些结果与大肠不同节段具有不同功能这一理论相符。
