Ene Cosmin, Nicolae Ilinca, Ene Corina Daniela
Department of Urology, 'Carol Davila' University of Medicine and Pharmacy, 050474 Bucharest, Romania.
Department of Urology, 'St. John' Clinical Hospital of Emergency, 042122 Bucharest, Romania.
Exp Ther Med. 2023 Sep 1;26(4):483. doi: 10.3892/etm.2023.12182. eCollection 2023 Oct.
The present paper aimed to investigate the altered angiogenetic mechanisms in hypoxic conditions in patients with prostate tumours, in correlation with common clinicopathologic variables. A case-control study was developed and included 87 patients with prostate tumours [40 diagnosed with benign prostatic hyperplasia (BPH) and 47 diagnosed with prostate cancer (PCa), using prostate transrectal biopsy] and 40 healthy subjects. The following parameters were evaluated in the serum of volunteers: Hypoxia-inducible factor (HIF)-1α, fibroblast growth factor (FGF)-2, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2 and -9, thrombospondin (TSP)-1 and soluble VEGF-1 receptor. Experimental data analysis demonstrated increasing amounts of inflammation in patients with PCa (IL-6, 18.1±4.7 ng/ml) and BPH (IL-6, 16.3±5.1 ng/ml) vs. control (IL-6, 4.1±1.2 ng/ml); overregulation of HIF1α in patients with PCa (129.3±21.8 ng/ml) compared with patients with BPH (65.6±18.2 ng/ml) and control (61.3±12.7 ng/ml); angiogenesis abnormalities in patients with PCa (upregulation of FGF-2, VEGF, MMP-2 and -9, suppression of TSP-1 and soluble VEGR-1) and BPH (upregulation FGF-2 and VEGF) compared with the control group. In conclusion, a greater understanding of the biological mechanism, the pathological roles and the clinical significance of various proangiogenic parameters and angiogenic-suppressor proteins seem useful in clinical practice for establishing an early diagnosis of prostate pathology and finding an individualized therapeutic approach.
本研究旨在探讨前列腺肿瘤患者在缺氧条件下血管生成机制的改变，并与常见临床病理变量相关联。开展了一项病例对照研究，纳入87例前列腺肿瘤患者[经直肠前列腺活检确诊40例良性前列腺增生（BPH）和47例前列腺癌（PCa）]以及40名健康受试者。对志愿者血清中的以下参数进行了评估：缺氧诱导因子（HIF）-1α、成纤维细胞生长因子（FGF）-2、血管内皮生长因子（VEGF）、基质金属蛋白酶（MMP）-2和-9、血小板反应蛋白（TSP）-1以及可溶性VEGF-1受体。实验数据分析表明，与对照组（IL-6，4.1±1.2 ng/ml）相比，PCa患者（IL-6，18.1±4.7 ng/ml）和BPH患者（IL-6，16.3±5.1 ng/ml）的炎症水平升高；与BPH患者（65.6±18.2 ng/ml）和对照组（61.3±12.7 ng/ml）相比，PCa患者的HIF1α上调（129.3±21.8 ng/ml）；与对照组相比，PCa患者（FGF-2、VEGF、MMP-2和-9上调，TSP-1和可溶性VEGR-1受抑制）和BPH患者（FGF-2和VEGF上调）存在血管生成异常。总之，深入了解各种促血管生成参数和血管生成抑制蛋白的生物学机制、病理作用及临床意义，对于在临床实践中早期诊断前列腺病变及找到个体化治疗方法似乎是有用的。
