医院获得性感染患者中细菌对黏菌素和多重耐药的分子机制。
Molecular mechanisms of colistin- and multidrug-resistance in bacteria among patients with hospital-acquired infections.
作者信息
Khurana Surbhi, Katiyar Amit, Puraswani Mamta, Sharma Divya, Walia Kamini, Malhotra Rajesh, Mathur Purva
机构信息
Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi.
Centralized Core Research Facility, Bioinformatics Facility, All India Institute of Medical Sciences, New Delhi, India.
出版信息
Future Sci OA. 2023 Aug 28;9(10):FSO896. doi: 10.2144/fsoa-2022-0055. eCollection 2023 Dec.
AIM
The increasing burden of resistance in Gram-negative bacteria (GNB) is becoming a major issue for hospital-acquired infections. Therefore, understanding the molecular mechanisms is important.
METHODOLOGY
Resistance genes of phenotypically colistin-resistant GNB (n = 60) were determined using whole genome sequencing. Antimicrobial susceptibility patterns were detected by Vitek®2 & broth microdilution.
RESULTS
Of these phenotypically colistin-resistant isolates, 78% were also genetically resistant to colistin. Activation of efflux pumps, and point-mutations in , and genes conferred colistin resistance among GNB. Eight different strains of were identified and ST43 was the most prominent strain with capsular type-specific (cps) gene KL30.
DISCUSSION
These results, in combination with rapid diagnostic methods, will help us better advice appropriate antimicrobial regimens.
目的
革兰氏阴性菌（GNB）耐药负担的不断增加正成为医院获得性感染的一个主要问题。因此，了解其分子机制很重要。
方法
使用全基因组测序确定表型耐黏菌素GNB（n = 60）的耐药基因。通过Vitek®2和肉汤微量稀释法检测抗菌药物敏感性模式。
结果
在这些表型耐黏菌素的分离株中，78%在基因上也对黏菌素耐药。外排泵的激活以及 、 和 基因中的点突变赋予了GNB对黏菌素的耐药性。鉴定出8种不同的 菌株，ST43是具有荚膜型特异性（cps）基因KL30的最主要菌株。
讨论
这些结果与快速诊断方法相结合，将有助于我们更好地建议合适的抗菌治疗方案。
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