School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Liaoning Provincial Aquatic Products Deep Processing Technology Research Center, Dalian 116034, P. R. China.
Jiangsu Palarich Food Co., Ltd., Xuzhou 221116, P. R. China.
J Agric Food Chem. 2023 Oct 11;71(40):14626-14637. doi: 10.1021/acs.jafc.3c03853. Epub 2023 Sep 27.
Our previous study reported that the marine dietary bioactive compound fucoxanthin (FX) has the potential to reduce the level of oxidation in retinal Müller cells (RMCs) induced by ultraviolet B (UVB) irradiation. However, the gastrointestinal environment can inhibit the bioavailability and absorption of FX in the cell systems. In the current study, FX was initially digested in a simulated gastrointestinal fluid. Nine main digestive products were identified, and the photoprotective activities of FX simulated gastrointestinal digestion products (FX-ID) were assessed in the same RMC model. FX-ID significantly reduced intracellular ROS and alleviated apoptosis. Western blot assays showed that FX-ID inhibited phosphorylated proteins in the MAPK and NF-κB signaling pathways. Our proteomics analysis revealed that the differentially expressed proteins were linked to biological networks associated with antioxidation and metabolic processes. The data may provide insight into the photoprotective mechanisms of FX-ID and promote the development of various functional foods to prevent retinal disorders.
我们之前的研究表明，海洋膳食生物活性化合物岩藻黄质（FX）具有降低紫外线 B（UVB）照射诱导的视网膜 Müller 细胞（RMC）氧化水平的潜力。然而，胃肠道环境会抑制 FX 在细胞系统中的生物利用度和吸收。在本研究中，FX 首先在模拟的胃肠道液中进行消化。鉴定出 9 种主要的消化产物，并在相同的 RMC 模型中评估 FX 模拟胃肠道消化产物（FX-ID）的光保护活性。FX-ID 显著降低了细胞内 ROS 并减轻了细胞凋亡。Western blot 检测结果表明，FX-ID 抑制了 MAPK 和 NF-κB 信号通路中的磷酸化蛋白。我们的蛋白质组学分析表明，差异表达的蛋白质与与抗氧化和代谢过程相关的生物网络有关。这些数据可能为 FX-ID 的光保护机制提供了深入的了解，并促进了各种预防视网膜疾病的功能性食品的开发。
