Shi Yixin, Ren Jiaying, Zhao Baomin, Zhu Taihai, Qi Hang
School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Liaoning Provincial Aquatic Products Deep Processing Technology Research Center, Dalian 116034, P. R. China.
Jiangsu Palarich Food Co., Ltd., Xuzhou 221116, P. R. China.
J Agric Food Chem. 2022 Mar 16;70(10):3181-3193. doi: 10.1021/acs.jafc.1c07980. Epub 2022 Feb 24.
Long-term exposure to sunlight and/or blue light causes vision damage to people of all ages. Dietary pigments and polyphenols have been shown to have photoprotective potential for eyes; however, many unknowns regarding the protective mechanism remain. In this study, we used ultraviolet B (UVB) irradiation-induced retinal Müller cells (RMCs) to screen for dietary polyphenols and pigment compounds with effective photoprotective activity. Fucoxanthin (FX) was shown to have the best therapeutic effect, and the mechanism was evaluated lipidomics analysis. Both intra- and extracellular ROS, mitochondrial depolarization, and DNA damage induced by UVB irradiation were inhibited by FX. Meanwhile, FX modulated the MAPK signaling pathway, which is correlated with apoptosis and inflammation. Our lipidomics data revealed that FX regulated lipid metabolism disorder and protected the membrane structure. These results confirm the effective photoprotective effects of FX, which may lead to new insights into FX-functionalized photoprotective foods.
长期暴露在阳光和/或蓝光下会对所有年龄段的人造成视力损害。膳食色素和多酚已被证明对眼睛具有光保护潜力；然而，关于其保护机制仍有许多未知之处。在本研究中，我们使用紫外线B（UVB）照射诱导的视网膜穆勒细胞（RMCs）来筛选具有有效光保护活性的膳食多酚和色素化合物。岩藻黄质（FX）显示出最佳治疗效果，并通过脂质组学分析评估其机制。FX抑制了UVB照射诱导的细胞内和细胞外活性氧、线粒体去极化和DNA损伤。同时，FX调节了与细胞凋亡和炎症相关的丝裂原活化蛋白激酶（MAPK）信号通路。我们的脂质组学数据表明，FX调节脂质代谢紊乱并保护膜结构。这些结果证实了FX有效的光保护作用，这可能为FX功能化的光保护食品带来新的见解。
