National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow G1 1RD, UK.
Biosensors (Basel). 2023 Sep 8;13(9):876. doi: 10.3390/bios13090876.
This study presents the development of a portable fluorometer with a smartphone application designed to facilitate the early screening of chronic kidney and renal diseases by enabling the sensitive detection of urinary albumin. Utilizing a fluorescence-based aptasensor, the device achieved a linear calibration curve (0.001-1.5 mg/mL) with a linearity of up to 0.98022 and a detection limit of 0.203 µg/mL for human serum albumin (HSA). The analysis of 130 urine samples demonstrated comparable performance between this study's fluorometer, a commercial fluorometer, and the standard automated method. These findings validate the feasibility of the portable fluorometer and aptasensor combination as a reliable instrument for the sensitive and specific measurement of HSA in urine samples. Moreover, the fluorometer's portability offers potential applications in portable point-of-care testing, enhancing its utility in clinical settings for early disease screening.
本研究开发了一种带有智能手机应用程序的便携式荧光计，旨在通过敏感检测尿液中的白蛋白，方便早期筛查慢性肾病和肾脏疾病。该设备利用基于荧光的适体传感器，实现了线性校准曲线（0.001-1.5mg/mL），线性度高达 0.98022，人血清白蛋白（HSA）的检测限为 0.203µg/mL。对 130 个尿液样本的分析表明，该研究的荧光计与商用荧光计和标准自动化方法之间具有相当的性能。这些发现验证了便携式荧光计和适体传感器组合作为一种可靠仪器，用于敏感和特异性测量尿液样本中 HSA 的可行性。此外，荧光计的便携性为便携式即时检测提供了潜在的应用，增强了其在临床环境中用于早期疾病筛查的实用性。
