Department of Electrical Engineering, AmirKabir University of Technology, Tehran, Iran.
MEMS and NEMS Engineering Department, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
IET Nanobiotechnol. 2020 Apr;14(2):126-132. doi: 10.1049/iet-nbt.2018.5165.
In this work, the authors reported the hybrid architecture of carbon nanotube (CNT)-zinc oxide (ZnO) nanowire as a multi-functional probe in amperometric immunosensor for the detection of urine albumin. Low-cost substrate such as glass is possible because of novel low-temperature growth process of CNT/ZnO nanowires as a multi-function electrode in this sensor. Based on Schottky like behaviour this structure exhibit excellent high current density to achieve higher performance. Measurement of urine albumin is a new way for early detection of diabetic and also low concentration of it in culture media is also considered in order to verify the conversion of stem cells to liver cells. Human albumin serum antibody is used as a selective and sensitive part. The amperometric performance of immunosensor is studied and showed excellent performance for detection of albumin in urine samples. Very high linear range (from 3.3 ng/µl to 3.3 mg/µl) with a correlation coefficient of 0.825 and low detection limit (3.3 ng/µl or 4.96 × 10 mol l) are the main characteristics of this sensor. Due to the high dynamic range and sensitivity, this sensor was also used in medical diagnosis and biomedical applications.
在这项工作中，作者报道了碳纳米管 (CNT)-氧化锌 (ZnO) 纳米线的混合结构作为一种多功能探针，用于安培免疫传感器中检测尿液白蛋白。由于 CNT/ZnO 纳米线的新型低温生长工艺可作为传感器中的多功能电极，因此有可能使用低成本的基底，如玻璃。基于肖特基行为，这种结构表现出优异的高电流密度，从而实现更高的性能。测量尿液白蛋白是早期检测糖尿病的新方法，同时也考虑了培养基中白蛋白的低浓度，以验证干细胞向肝细胞的转化。人白蛋白血清抗体被用作选择性和灵敏的部分。研究了免疫传感器的安培性能，结果表明该传感器在检测尿液样本中的白蛋白方面表现出优异的性能。该传感器具有非常高的线性范围（从 3.3ng/µl 到 3.3mg/µl，相关系数为 0.825）和低检测限（3.3ng/µl 或 4.96 × 10-9mol l），是该传感器的主要特点。由于具有高动态范围和灵敏度，该传感器还用于医学诊断和生物医学应用。
