Reynolds Jocelyn, Loeffler Reid S, Leigh Preston J, Lopez Hannah A, Yoon Jeong-Yeol
Department of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA.
Department of Neuroscience, The University of Arizona, Tucson, AZ 85721, USA.
Biosensors (Basel). 2023 Sep 15;13(9):885. doi: 10.3390/bios13090885.
Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify nucleic acid amplification. Recently, numerous attempts have been made to incorporate paper microfluidics into these isothermal amplification tests. Paper microfluidics (including lateral flow strips) have been used to extract nucleic acids, amplify the target gene, and detect amplified products, all toward automating the process. We investigated the literature from 2020 to the present, i.e., since the onset of the COVID-19 pandemic, during which a significant surge in isothermal amplification tests has been observed. Paper microfluidic detection has been used extensively for recombinase polymerase amplification (RPA) and its related methods, along with loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA). Detection was conducted primarily with colorimetric and fluorometric methods, although a few publications demonstrated flow distance- and surface-enhanced Raman spectroscopic (SERS)-based detection. A good number of publications could be found that demonstrated both amplification and detection on paper microfluidic platforms. A small number of publications could be found that showed extraction or all three procedures (i.e., fully integrated systems) on paper microfluidic platforms, necessitating the need for future work.
等温核酸扩增检测最近比聚合酶链反应（PCR）更受欢迎，因为它们只需要恒定温度，并且显著简化了核酸扩增过程。最近，人们进行了许多尝试，将纸基微流控技术应用于这些等温扩增检测中。纸基微流控技术（包括侧向流动试纸条）已被用于提取核酸、扩增目标基因以及检测扩增产物，所有这些都是为了实现该过程的自动化。我们研究了2020年至今的文献，即自新冠疫情爆发以来，在此期间等温扩增检测出现了显著增长。纸基微流控检测已广泛用于重组酶聚合酶扩增（RPA）及其相关方法，以及环介导等温扩增（LAMP）和滚环扩增（RCA）。检测主要采用比色法和荧光法，不过也有一些出版物展示了基于流动距离和表面增强拉曼光谱（SERS）的检测方法。可以找到大量在纸基微流控平台上同时进行扩增和检测的出版物。也能找到少数在纸基微流控平台上展示提取或所有三个步骤（即完全集成系统）的出版物，这表明未来仍有工作需要开展。
