Hu Qingxi, Huang Zhenwei, Zhang Haiguang, Ramalingam Murugan
Rapid Manufacturing Engineering Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200444, China.
Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai 200072, China.
Biomimetics (Basel). 2023 Sep 11;8(5):419. doi: 10.3390/biomimetics8050419.
The coaxial electrospinning process has been widely used in the biomedical field, and its process parameters affect product quality seriously. In this paper, the influence of key process parameters of coaxial electrostatic spinning (solution concentration, electrospinning voltage, acceptance distance and liquid supply velocity) on the preparation of a membrane with Chitosan, Polyethylene oxide and nano-silver as the core layer and Polycaprolactone as the shell layer was studied. The optimal combination of key process parameters was obtained by using an orthogonal test, scanning electron microscope, transmission electron microscope and macro-characterization diagram. The results showed that the coaxial electrospun membrane had good mechanical properties (tensile strength is about 2.945 Mpa), hydrophilicity (the water contact angle is about 72.28°) and non-cytotoxicity, which was conducive to cell adhesion and proliferation. The coaxial electrospun membrane with nano-silver has an obvious inhibitory effect on Escherichia coli and Staphylococcus aureus. In summary, the coaxial electrospun membrane that we produced is expected to be used in clinical medicine, such as vascular stent membranes and bionic blood vessels.
同轴静电纺丝工艺在生物医学领域已得到广泛应用，其工艺参数对产品质量有严重影响。本文研究了同轴静电纺丝的关键工艺参数（溶液浓度、静电纺丝电压、接收距离和供液速度）对以壳聚糖、聚环氧乙烷和纳米银为芯层、聚己内酯为壳层的膜制备的影响。通过正交试验、扫描电子显微镜、透射电子显微镜和宏观表征图获得了关键工艺参数的最佳组合。结果表明，同轴静电纺丝膜具有良好的力学性能（拉伸强度约为2.945 Mpa）、亲水性（水接触角约为72.28°）和无细胞毒性，有利于细胞黏附和增殖。含纳米银的同轴静电纺丝膜对大肠杆菌和金黄色葡萄球菌有明显的抑制作用。综上所述，我们制备的同轴静电纺丝膜有望用于临床医学，如血管支架膜和仿生血管。
