Smoczer Cristine, Yuth Kenneth R, Askar Mazin A, Young Laura A, Paurazas Susan B
Division of Integrated Biomedical Sciences, University of Detroit Mercy School of Dentistry, Detroit, MI 48208, USA.
Graduate Endodontics, University of Detroit Mercy School of Dentistry, Detroit, MI 48208, USA.
Dent J (Basel). 2023 Sep 19;11(9):220. doi: 10.3390/dj11090220.
Advanced platelet-rich fibrin (A-PRF) provides the scaffold and growth factors necessary for stem cells to proliferate and differentiate in successful regenerative endodontic procedures. This study investigates the release of transforming growth factor-β1 (TGF-β1), platelet-derived growth factor (PDGF), and vascular endothelial growth factor (VEGF) from A-PRF in cell culture media in the presence and absence of mineral trioxide aggregate (MTA) or Biodentine. Additionally, this research assesses the viability and migration of stem cells of the apical papilla (SCAP) in previously conditioned media. A-PRF obtained from 14 participants were incubated for 7 days in cell culture media alone or via layering with MTA or Biodentine discs and the release of selected growth factors in the media was evaluated using ELISA. The viability of SCAP grown in conditioned media was measured using the CCK8 assay, while SCAP migration was assessed via a transwell assay by counting migrated cells. The release of TGF-β1, PDGF, and VEGF was significantly higher in media with A-PRF alone than in the presence of either calcium-based silicate material ( < 0.05), which showed no difference from the no-A-PRF control ( < 0.05). None of the tested growth factors released in the A-PRF-conditioned media correlated with clot weight. A-PRF-conditioned media, both with and without calcium-based silicate materials, did not impact SCAP viability and migration ( > 0.05). This study shows that SCAP behavior is not impacted by the decrease in growth factor released in the presence of calcium-based silicate materials and that their role in REPs warrants further investigation.
高级富血小板纤维蛋白（A-PRF）为干细胞在成功的再生性牙髓治疗程序中增殖和分化提供了必要的支架和生长因子。本研究调查了在有和没有三氧化矿物凝聚体（MTA）或生物陶瓷的情况下，A-PRF在细胞培养基中转化生长因子-β1（TGF-β1）、血小板衍生生长因子（PDGF）和血管内皮生长因子（VEGF）的释放情况。此外，本研究评估了在预先处理过的培养基中根尖乳头干细胞（SCAP）的活力和迁移能力。从14名参与者获取的A-PRF分别在单独的细胞培养基中孵育7天，或与MTA或生物陶瓷圆盘分层孵育，然后使用酶联免疫吸附测定法（ELISA）评估培养基中所选生长因子的释放情况。使用CCK8测定法测量在处理过的培养基中生长的SCAP的活力，而通过计数迁移细胞，通过Transwell测定法评估SCAP的迁移情况。单独使用A-PRF的培养基中TGF-β1、PDGF和VEGF的释放明显高于存在任何一种钙基硅酸盐材料的情况（P<0.05），这与无A-PRF对照组无差异（P<0.05）。A-PRF处理过的培养基中释放的所有测试生长因子均与凝块重量无关。有和没有钙基硅酸盐材料的A-PRF处理过的培养基均不影响SCAP的活力和迁移（P>0.05）。本研究表明，钙基硅酸盐材料存在时生长因子释放的减少不会影响SCAP的行为，其在再生性牙髓治疗中的作用值得进一步研究。
