Ballal Nidambur Vasudev, Narkedamalli Rajkumar, Ruparel Nikita B, Shenoy Padmaja A, Bhat Vinutha R, Belle Vijetha Shenoy
Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
J Endod. 2022 May;48(5):620-624. doi: 10.1016/j.joen.2022.02.007. Epub 2022 Feb 22.
Chemically released growth factors play a vital role in regenerative endodontics. Transforming growth factor beta 1 (TGF-β1) is 1 of the most extensively studied bioactive molecules that promotes cell proliferation, differentiation, and chemotaxis. The goal of the current research was to analyze the effect of 7% maleic acid (MA) root conditioning of an infected root canal on the release of TGF-β1.
Single-rooted human teeth were decoronated, and the canals were enlarged with a Peeso reamer. The samples were divided into biofilm and nonbiofilm groups. Subsequently, all the samples of both groups were flushed with 10 mL of each irrigant, namely, 1.5% sodium hypochlorite (NaOCl), 7% MA, 17% EDTA, and a combination of 1.5% NaOCl with 17% EDTA or 7% MA, for 10 minutes each. TGF-β1 was estimated quantitatively using an enzyme-linked immunosorbent assay kit.
TGF-β1 release was lowest among the biofilm samples compared with nonbiofilm among all the groups. MA 7% with 1.5% NaOCl rendered higher amounts of growth factor release in contrast to the combination of 17% EDTA and 1.5% NaOCl in both the biofilm and nonbiofilm groups (P < .048). The nonbiofilm samples treated with 7% MA alone illustrated higher growth factor release compared with 17% EDTA only (P < .006), but there was no significant difference in growth factor release among the biofilm samples treated with 7% MA and 17% EDTA.
Bacterial biofilms modified the release of TGF-β1. MA 7% was observed to be significantly more efficacious than 17% EDTA in TGF-β1 growth factor release from radicular dentin.
化学释放的生长因子在再生牙髓治疗中起着至关重要的作用。转化生长因子β1（TGF-β1）是研究最广泛的促进细胞增殖、分化和趋化性的生物活性分子之一。本研究的目的是分析感染根管7%马来酸（MA）根管预处理对TGF-β1释放的影响。
拔除单根人牙，用Peeso扩孔钻扩大根管。将样本分为生物膜组和非生物膜组。随后，两组的所有样本均用10 mL每种冲洗液冲洗10分钟，冲洗液分别为1.5%次氯酸钠（NaOCl）、7% MA、17%乙二胺四乙酸（EDTA）以及1.5% NaOCl与17% EDTA或7% MA的组合。使用酶联免疫吸附测定试剂盒对TGF-β1进行定量评估。
与所有组中的非生物膜样本相比，生物膜样本中TGF-β1的释放量最低。在生物膜组和非生物膜组中，7% MA与1.5% NaOCl相比，17% EDTA与1.5% NaOCl组合能释放更高量的生长因子（P < 0.048）。仅用7% MA处理的非生物膜样本比仅用17% EDTA处理的样本显示出更高的生长因子释放量（P < 0.006），但在7% MA和17% EDTA处理的生物膜样本中，生长因子释放量没有显著差异。
细菌生物膜改变了TGF-β1的释放。观察到7% MA在从根管牙本质释放TGF-β1生长因子方面比17% EDTA显著更有效。
