Yang Kaining, Shen Lei, Zhang Lin, Sun Wenxin, Zou Yuhong, Ren Yande, Zeng Rongchang
Department of Bioengineering, College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao 266590, China.
Hospital of Shandong University of Science and Technology, Qingdao 266590, China.
J Funct Biomater. 2023 Sep 12;14(9):470. doi: 10.3390/jfb14090470.
(1) Background: Traditional dressings can only superficially cover the wound, they have widespread issues with inadequate bacterial isolation and liquid absorption, and it is simple to inflict secondary wound injury when changing dressings. Therefore, it is crucial for wound healing to develop a new kind of antimicrobial colloidal dressing with good antibacterial, hygroscopic, and biocompatible qualities. (2) Methods: Ag-montmorillonite/chitosan (Ag-MMT/CS) colloid, a new type of antibacterial material, was prepared from two eco-friendly materials-namely, montmorillonite and chitosan-as auxiliary materials, wherein these materials were mixed with the natural metal Ag, which is an antibacterial agent. The optimum preparation technology was explored, and Ag-MMT/CS was characterized. Next, Staphylococcus aureus, which is a common skin infection bacterium, was considered as the experimental strain, and the in vitro antibacterial activity and cytocompatibility of the Ag-MMT/CS colloid were investigated through various experiments. Subsequently, a rat skin infection model was established to explore the in vivo antibacterial effect. (3) Results: In vitro studies revealed that the Ag-MMT/CS colloid had a good antibacterial effect on , with an inhibition zone diameter of 18 mm and an antibacterial rate of 99.18%. After co-culture with cells for 24 h and 72 h, the cell survival rates were 88% and 94%, respectively. The cells showed normal growth and proliferation, and no evident dead cells were observed under the laser confocal microscope. After applying the colloid to the rat skin infection model, the Ag-MMT/CS treatment group exhibited faster wound healing and better local exudation and absorption in the wound than the control group, suggesting that the Ag-MMT/CS colloid exhibited a better antibacterial effect on the . (4) Conclusions: Ag, chitosan, and MMT present in the Ag-MMT/CS colloid dressing exert synergistic effects, and it has good antibacterial effects, cytocompatibility, and hygroscopicity, indicating that this colloid has the potential to become a next-generation clinical antibacterial dressing.
(1) 背景：传统敷料仅能表面覆盖伤口，存在细菌隔离不足和液体吸收不佳等普遍问题，且换药时易造成二次伤口损伤。因此，开发一种具有良好抗菌、吸湿和生物相容性的新型抗菌胶体敷料对伤口愈合至关重要。(2) 方法：以蒙脱石和壳聚糖这两种环保材料为辅助材料，与抗菌剂天然金属银混合，制备新型抗菌材料银 - 蒙脱石/壳聚糖（Ag - MMT/CS）胶体。探索了最佳制备工艺，并对Ag - MMT/CS进行了表征。接下来，以常见皮肤感染细菌金黄色葡萄球菌为实验菌株，通过各种实验研究Ag - MMT/CS胶体的体外抗菌活性和细胞相容性。随后，建立大鼠皮肤感染模型以探究体内抗菌效果。(3) 结果：体外研究表明，Ag - MMT/CS胶体对[具体细菌名称未给出]具有良好的抗菌效果，抑菌圈直径为18 mm，抗菌率为99.18%。与细胞共培养24 h和72 h后，细胞存活率分别为88%和94%。细胞生长和增殖正常，激光共聚焦显微镜下未观察到明显死细胞。将胶体应用于大鼠皮肤感染模型后，Ag - MMT/CS治疗组伤口愈合速度比对照组快，伤口局部渗出和吸收情况更好，表明Ag - MMT/CS胶体对[具体细菌名称未给出]具有更好的抗菌效果。(4) 结论：Ag - MMT/CS胶体敷料中的银、壳聚糖和蒙脱石发挥协同作用，具有良好的抗菌效果、细胞相容性和吸湿性，表明该胶体有潜力成为下一代临床抗菌敷料。
