Xu Na, Yuan Yucheng, Ding Liangping, Li Jiangfeng, Jia Jiezhi, Li Zheng, He Dengfeng, Yu Yunlong
Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.
School of Materials science and Engineering, Xihua University, No.999, Jinzhou Road, Jinniu District, Chengdu City, Sichuan Province, Chengdu, 610039, China.
Burns Trauma. 2022 Jul 27;10:tkac019. doi: 10.1093/burnst/tkac019. eCollection 2022.
Most traditional wound dressings only partially meet the needs of wound healing because of their single function. Patients usually suffer from the increasing cost of treatment and pain resulting from the frequent changing of wound dressings. Herein, we have developed a mutifunctional cryogel to promote bacterial infected wound healing based on a biocompatible polysaccharide.
The multifunctional cryogel is made up of a compositive scaffold of chitosan (CS), gelatin (Gel) and tannic acid (TA) and formed silver nanoparticles (Ag NPs). A liver bleeding rat model was used to evaluate the dynamic hemostasis performance of the various cryogels. In order to evaluate the antibacterial properties of the prepared cryogels, gram-positive bacterium () and gram-negative bacterium () were cultured with the cryogels for 12 h. Meanwhile, was introduced to cause bacterial infection . After treatment for 2 days, the exudates from wound sites were dipped for bacterial colony culture. Subsequently, the anti-inflammatory effect of the various cryogels was evaluated by western blotting and enzyme-linked immunosorbent assay. Finally, full-thickness skin defect models on the back of SD rats were established to assess the wound healing performances of the cryogels.
Due to its porous structure, the multifunctional cryogel showed fast liver hemostasis. The introduced Ag NPs endowed the cryogel with an antibacterial efficiency of >99.9% against both and . Benefited from the polyphenol groups of TA, the cryogel could inhibit nuclear factor-κB nuclear translocation and down-regulate inflammatory cytokines for an anti-inflammatory effect. Meanwhile, excessive reactive oxygen species could also be scavenged effectively. Despite the presence of Ag NPs, the cryogel did not show cytotoxicity and hemolysis. Moreover, experiments demonstrated that the biocompatible cryogel displayed effective bacterial disinfection and accelerated wound healing.
The multifunctional cryogel, with fast hemostasis, antibacterial and anti-inflammation properties and the ability to promote cell proliferation could be widely applied as a wound dressing for bacterial infected wound healing.
大多数传统伤口敷料由于功能单一，只能部分满足伤口愈合的需求。患者通常会因频繁更换伤口敷料而承受治疗费用增加和疼痛的困扰。在此，我们基于一种生物相容性多糖开发了一种多功能冷冻凝胶，以促进细菌感染伤口的愈合。
多功能冷冻凝胶由壳聚糖（CS）、明胶（Gel）和单宁酸（TA）的复合支架以及形成的银纳米颗粒（Ag NPs）组成。使用肝出血大鼠模型评估各种冷冻凝胶的动态止血性能。为了评估所制备冷冻凝胶的抗菌性能，将革兰氏阳性菌（）和革兰氏阴性菌（）与冷冻凝胶共培养12小时。同时，引入（此处原文似乎不完整）以引起细菌感染。治疗2天后，采集伤口部位的渗出液进行细菌菌落培养。随后，通过蛋白质免疫印迹法和酶联免疫吸附测定法评估各种冷冻凝胶的抗炎效果。最后，在SD大鼠背部建立全层皮肤缺损模型，以评估冷冻凝胶的伤口愈合性能。
由于其多孔结构，多功能冷冻凝胶显示出快速的肝脏止血效果。引入的Ag NPs使冷冻凝胶对和的抗菌效率均>99.9%。受益于TA的多酚基团，冷冻凝胶可抑制核因子-κB核转位并下调炎性细胞因子，从而发挥抗炎作用。同时，过量的活性氧也能被有效清除。尽管存在Ag NPs，但冷冻凝胶未表现出细胞毒性和溶血现象。此外，（此处原文似乎不完整）实验表明，这种生物相容性冷冻凝胶具有有效的细菌消毒作用并能加速伤口愈合。
这种具有快速止血、抗菌、抗炎特性以及促进细胞增殖能力的多功能冷冻凝胶可广泛应用于细菌感染伤口愈合的伤口敷料。
