Kim Hyung Kyu, Ahn Dan Bi, Jang Han Byeol, Ma Jing, Xing Juping, Yoon Joo Won, Lee Kyung Hee, Lee Dong Min, Kim Chang Hyun, Kim Hee Young
Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Department of Oral Physiology, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of Korea.
J Funct Biomater. 2023 Sep 18;14(9):476. doi: 10.3390/jfb14090476.
Blood group mismatch in veterinary medicine is a significant problem in blood transfusion, sometimes leading to severe transfusion reactions and even patient death. Blood groups vary from species to species and there are three known blood groups in cats: A, B and AB. While A-type cats are most common, there is a shortage of feline B-type blood groups in cats. By using methoxy polyethylene glycol (mPEG) to protect antigenic epitopes on red blood cells (RBCs), we aimed to find the optimal conditions for the production of feline universal RBCs. The surfaces of feline A-type RBCs were treated with mPEG at various molecular weights and concentrations. Agglutination tests showed that the coating of feline A-type RBCs with mPEG of 20 kDa and 2 mM blocked hemagglutination to feline anti-A alloantibodies over 8 h. While no differences in RBC size and shape between intact and mPEG-treated RBCs were seen, coating RBCs with mPEG inhibited the binding of feline anti-A alloantibodies. Furthermore, the mPEG-treated RBCs did not cause spontaneous hemolysis or osmotic fragility, compared to control RBCs. According to a monocyte monolayer assay, mPEG treatment significantly reduced feline anti-A antibody-mediated phagocystosis of RBCs. These results confirm the potential of using activated mPEG on feline A-type RBC to create universal erythrocytes for transfusion to B-type cats.
兽医医学中的血型不匹配是输血中的一个重大问题，有时会导致严重的输血反应甚至患者死亡。不同物种的血型各不相同，猫有三种已知血型：A、B和AB。虽然A型猫最为常见，但猫中B型血型短缺。通过使用甲氧基聚乙二醇（mPEG）来保护红细胞（RBC）上的抗原表位，我们旨在找到生产猫通用红细胞的最佳条件。用不同分子量和浓度的mPEG处理猫A型红细胞表面。凝集试验表明，用20 kDa和2 mM的mPEG包被猫A型红细胞可在8小时以上阻断对猫抗A同种抗体的血凝反应。虽然完整红细胞和mPEG处理的红细胞在大小和形状上没有差异，但用mPEG包被红细胞会抑制猫抗A同种抗体的结合。此外，与对照红细胞相比，mPEG处理的红细胞不会引起自发性溶血或渗透脆性。根据单核细胞单层试验，mPEG处理显著降低了猫抗A抗体介导红细胞的吞噬作用。这些结果证实了在猫A型红细胞上使用活化的mPEG来制造通用红细胞用于输血给B型猫的潜力。
