Jeong S T, Byun S M
Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Taejeon, Korea.
Artif Cells Blood Substit Immobil Biotechnol. 1996 Sep;24(5):503-11. doi: 10.3109/10731199609117442.
Human Rb(+) AB type red blood cells were modified with methoxy-polyethylene glycol (mPEG) in order to decrease agglutinabilities toward clinically important anti-A, anti-B and anti-D reagents. Attachment of mPEG to membrane proteins of the red blood cell was identified by the shift of well-known blood group active glycoprotein bands such as band 3, 4.5 and PAS-1 on the SDS-polyacrylamide gel electrophoresis. When 4mM concentration of mPEG was added to red blood cells, agglutination was minimum by a blood group typing and microwell agglutination tests. Antibody binding tests showed decreased antibody binding to blood group antigens after mPEG attachment. The decrease of both agglutinability and antibody binding was the result of mPEG attachment to blood group active glycoprotein of the cell membrane. The morphology of red blood cells after mPEG attachment was the usual discocytic cell. Oxygen equilibrium curves of the mPEG-attached red blood cells were similar to unmodified red blood cells. This approach to decrease agglutinability of the red blood cells toward blood group antibodies may be used to develop a universally transfusible blood substitute.
人Rb(+) AB型红细胞用甲氧基聚乙二醇（mPEG）进行修饰，以降低其对临床上重要的抗A、抗B和抗D试剂的凝集性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上著名的血型活性糖蛋白条带（如带3、4.5和PAS-1）的迁移，鉴定了mPEG与红细胞膜蛋白的结合。当向红细胞中加入4mM浓度的mPEG时，血型分型和微孔凝集试验显示凝集作用最小。抗体结合试验表明，mPEG附着后抗体与血型抗原的结合减少。凝集性和抗体结合的降低是mPEG附着于细胞膜血型活性糖蛋白的结果。mPEG附着后红细胞的形态为常见的盘状细胞。mPEG修饰的红细胞的氧平衡曲线与未修饰的红细胞相似。这种降低红细胞对血型抗体凝集性的方法可用于开发通用的可输血替代品。
