Zapata-Zapata Carolina, Rojas-López Mauricio, García Liliana T, Quintero Wendy, Terrón María C, Luque Daniel, Mesa-Arango Ana C
Academic Group of Epidemiology, Faculty of Medicine, University of Antioquia, Medellín 050010, Colombia.
Group of Cellular Immunology and Immunogenetics (GICIG), Faculty of Medicine, University of Antioquia, Medellín 050010, Colombia.
J Fungi (Basel). 2023 Aug 30;9(9):888. doi: 10.3390/jof9090888.
is one of the most pathogenic species within the genus. Increased antifungal resistance has been reported, which is in part due to the organism's ability to form biofilms. In natural products derived from plants, such as essential oils (EOs) or their major components, there is significant potential to develop new antifungals or to both enhance the efficacy and reduce the toxicity of conventional antifungals. This study aimed to evaluate the effect of combining an EO of or thymol with fluconazole on an azole-resistant strain. Synergism was observed in the combination of fluconazole with the EO and with thymol, and minimal inhibitory concentrations for fluconazole decreased at least 32-fold. As a consequence of the synergistic interactions, mitochondrial membrane potential was reduced, and mitochondrial superoxide production increased. Alteration in nuclear morphology, cell surface, and ultrastructure was also observed. In conclusion, the synergistic interaction between EO or thymol with fluconazole reverted the azole-resistant phenotype. These findings suggest that EO or thymol alone, or in combination with fluconazole, have the potential for development as antifungal therapies for this yeast, including resistant strains.
是该属中致病性最强的物种之一。已有报道其抗真菌耐药性增加，部分原因是该生物体形成生物膜的能力。在源自植物的天然产物中，如精油（EOs）或其主要成分，开发新型抗真菌药物或提高传统抗真菌药物的疗效并降低其毒性具有巨大潜力。本研究旨在评估 或百里酚的精油与氟康唑联合使用对耐唑类 菌株的影响。在氟康唑与精油以及与百里酚的联合使用中观察到协同作用，氟康唑的最低抑菌浓度至少降低了32倍。由于协同相互作用，线粒体膜电位降低，线粒体超氧化物产生增加。还观察到核形态、细胞表面和超微结构的改变。总之， 精油或百里酚与氟康唑之间的协同相互作用逆转了耐唑类 表型。这些发现表明， 精油或百里酚单独使用或与氟康唑联合使用，有潜力开发成为针对这种酵母（包括耐药菌株）的抗真菌疗法。
