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采用振动切片机收获小鼠视交叉上核用于昼夜转录组分析。

Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis.

机构信息

Medical Research Council, Harwell Science Campus, Oxfordshire, UK.

Medical Research Council, Harwell Science Campus, Oxfordshire, UK; Wolfson Institute for Biomedical Research, University College London, London, UK.

出版信息

STAR Protoc. 2023 Dec 15;4(4):102618. doi: 10.1016/j.xpro.2023.102618. Epub 2023 Sep 26.

DOI:10.1016/j.xpro.2023.102618
PMID:37756154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10539961/
Abstract

The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe steps for mouse entrainment, isolation of the SCN, tissue preparation, slicing with a vibratome, and handling of the harvested SCN for RNA extraction. This protocol can also be used for harvesting other mammalian brain regions for genomic studies.

摘要

哺乳动物的视交叉上核（SCN）是主要的生物钟，它通过环境线索来协调日常的行为和生理反应。在这里，我们提供了一种通过振动切片机收获小鼠 SCN 进行昼夜转录组分析的方案。我们描述了小鼠驯化、SCN 分离、组织准备、振动切片以及收获的 SCN 用于 RNA 提取的步骤。该方案也可用于收获其他哺乳动物大脑区域进行基因组研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/c20db3168d1b/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/d44d63274ff1/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/4b9078e9d4e2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/280c1edf2987/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/f9a860c6d6b5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/c544b321e7b1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/ac9eaf70751c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/5b77c85b0fd9/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/c20db3168d1b/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/d44d63274ff1/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/4b9078e9d4e2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/280c1edf2987/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/f9a860c6d6b5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/c544b321e7b1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/ac9eaf70751c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/5b77c85b0fd9/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64de/10539961/c20db3168d1b/gr7.jpg

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