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建立用于食管鳞状细胞癌放射敏感性研究的患者来源异种移植瘤和类器官的方案。

Protocol to establish patient-derived xenograft and organoid for radiosensitivity of esophageal squamous cell carcinoma.

作者信息

Zhang Shaosen, Wang Caihong

机构信息

Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, China; Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, China.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103273. doi: 10.1016/j.xpro.2024.103273. Epub 2024 Sep 21.

DOI:10.1016/j.xpro.2024.103273
PMID:39306852
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11447403/
Abstract

Patient-derived xenograft (PDX) and organoids derived from PDX (PDXOs) are ideal pre-clinical models, which recapitulate the tumor heterogeneity. We provide the steps to establish PDX and PDXOs from patients with esophageal squamous cell carcinoma (ESCC) and their utility in examining radiotherapy. We describe steps for generating ESCC-PDXs, four cycles of ionizing radiation treatments using X-ray on PDX, and evaluating radiosensitivity of PDXs. We then detail procedures for establishing and characterizing radioresistant organoids derived from PDX. For complete details on the use and execution of this protocol, please refer to Liu et al., Yang et al., and Chen et al..

摘要

患者来源的异种移植瘤（PDX）以及源自PDX的类器官（PDXO）是理想的临床前模型，可概括肿瘤异质性。我们提供了从食管鳞状细胞癌（ESCC）患者建立PDX和PDXO的步骤及其在放射治疗检查中的应用。我们描述了生成ESCC-PDX的步骤、对PDX进行四个周期的X射线电离辐射治疗以及评估PDX的放射敏感性。然后，我们详细介绍了建立和表征源自PDX的放射抗性类器官的程序。有关本方案使用和执行的完整详细信息，请参考Liu等人、Yang等人和Chen等人的相关文献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/5214752d8170/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/3fd9286809e6/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/0b327dc91764/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/dd0b791bc1ac/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/4d00d64bce64/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/30ca0f0ebf1f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/4407cba19135/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/c07f4df67842/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/5214752d8170/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/3fd9286809e6/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/0b327dc91764/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/dd0b791bc1ac/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/4d00d64bce64/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/30ca0f0ebf1f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/4407cba19135/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/c07f4df67842/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7b7/11447403/5214752d8170/gr7.jpg

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本文引用的文献

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Cell Rep Med. 2024 Jun 18;5(6):101576. doi: 10.1016/j.xcrm.2024.101576. Epub 2024 May 21.
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Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer.
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VAV2 is required for DNA repair and implicated in cancer radiotherapy resistance.VAV2 对于 DNA 修复是必需的，并与癌症放射治疗抵抗有关。
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