Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo 208-0011, Japan; Department of Bacteriology, Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan; Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo 108-8641, Japan.
Antimicrobial Research Center, National Institute of Infectious Diseases, Tokyo 189-0002, Japan.
STAR Protoc. 2023 Dec 15;4(4):102556. doi: 10.1016/j.xpro.2023.102556. Epub 2023 Sep 26.
Infection with Helicobacter suis, which causes many cases of gastric disease, is not reliably diagnosed. Here, we present a protocol for detecting H. suis infection. We describe steps for collecting gastric biopsies and sera from patients, preparing DNA for PCR, and targeting the H. suis-specific gene. We then define procedures for inoculating biopsies onto primary agar plates and transferring colonies to secondary agar plates. Finally, we detail whole-genome sequencing of bacteria and assess H. suis infection in sera with ELISA. For complete details on the use and execution of these protocols, please refer to Matsui et al..
猪霍乱弧菌感染可引起多种胃病，但目前其诊断方法并不可靠。本研究介绍了一种猪霍乱弧菌感染检测方法。该方法包括采集胃活检组织和血清样本、提取 DNA 进行 PCR 扩增以及检测猪霍乱弧菌特异性基因。然后，我们描述了将活检组织接种到初级琼脂平板和将菌落转移到次级琼脂平板的步骤。最后，我们详细介绍了细菌全基因组测序和 ELISA 检测血清中猪霍乱弧菌感染的方法。有关这些方案的使用和实施的完整详细信息，请参考 Matsui 等人的研究。
