Department of Obstetrics and Gynecology, Hebei Medical University, Shijiazhuang, Hebei 050000, China; Department of Obstetrics and Gynecology, Shijiazhuang Maternity and Child Healthcare Hospital, Shijiazhuang, Hebei 050000, China.
College of Computer and Cyber Security, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
Genomics. 2023 Nov;115(6):110718. doi: 10.1016/j.ygeno.2023.110718. Epub 2023 Sep 25.
Endometriosis is a common gynecological disease with an enigmatic pathogenesis. This work explored the function of hsa_circ_0005991 in ovarian endometriosis. High-throughput RNA-Seq was conducted in five matched ectopic (EC) and eutopic (EU) samples. Further, several types of cell function experiments were conducted. According to bioinformatics analysis, a competing endogenous RNA network was established. It included 5 circRNAs, 13 miRNAs, and 551 mRNAs. The expression levels of hsa_circ_0005991 and Cdc42EP1 were significantly elevated, while miR-30b-3p was reduced in the EC group. Upregulation of hsa_circ_0005991 raised Cdc42EP1 levels, induced EMT, and boosted Ishikawa cell proliferation, migration, and invasion. hsa_circ_0005991 knockdown indicated the opposite effects. When co-transfected with miR-30b-3p mimics or inhibitors, these effects could be reversed, respectively. Western blot assays showed alterations of EMT markers in EC samples. hsa_circ_0005991/miR-30b-3p/Cdc42EP1 axis promotes the EMT process in endometriosis, which may offer a theoretical foundation for the mechanism exploration and therapy of this disease.
子宫内膜异位症是一种常见的妇科疾病，其发病机制尚不清楚。本研究探讨了 hsa_circ_0005991 在卵巢子宫内膜异位症中的作用。在五对异位（EC）和在位（EU）样本中进行了高通量 RNA-Seq 分析。此外，还进行了几种类型的细胞功能实验。根据生物信息学分析，建立了一个竞争性内源 RNA 网络，其中包括 5 个 circRNAs、13 个 miRNAs 和 551 个 mRNAs。在 EC 组中，hsa_circ_0005991 和 Cdc42EP1 的表达水平显著升高，而 miR-30b-3p 的表达水平降低。hsa_circ_0005991 的上调提高了 Cdc42EP1 的水平，诱导 EMT，并促进了 Ishikawa 细胞的增殖、迁移和侵袭。hsa_circ_0005991 的敲低则显示出相反的效果。当与 miR-30b-3p 模拟物或抑制剂共转染时，分别可以逆转这些效应。Western blot 检测显示 EC 样本中 EMT 标志物发生了改变。hsa_circ_0005991/miR-30b-3p/Cdc42EP1 轴促进了子宫内膜异位症中的 EMT 过程，这可能为该疾病的机制探索和治疗提供了理论基础。
