Hajisadeghi Samira, Rafiei Mehrad, Tahmasebi Elahe, Khafaei Mostafa
Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran.
School of Dentistry, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Mol Biol Rep. 2024 Jul 13;51(1):796. doi: 10.1007/s11033-024-09719-8.
Oral squamous cell carcinoma (OSCC) is a significant health issue worldwide, and the expression of long non-coding RNAs (lncRNAs) are altered in these malignancies. The present study evaluated the expression level of ATXN1 CDC42EP1 genes and the lncRNAs related to these genes (lnc-ATXN1L, lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1) in paraffin blocks of oral and pharyngeal squamous cell carcinoma (SCC) samples from patients referred to Amir Alam Hospital in Tehran, Iran.
This cross-sectional study was conducted on 76 paraffin blocks of oral and pharyngeal squamous cell carcinoma (SCC) samples from patients referred to Amir Alam Hospital in Tehran. The expression levels of ATXN1, CDC42EP1, lnc-ATXN1L, lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 were measured in all samples using a qPCR Master Mix kit. Real-time PCR was used to perform the reactions, and GAPDH was considered the housekeeping gene. Statistical analyses were conducted utilizing the Statistical Package for the Social Sciences (SPSS) version 22.0. The expression of lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 significantly differed between the two groups. All of them were downregulated (p < 0.05), and no significant difference was observed between the SCC samples and the adjacent tissue in other genes (p > 0.05). The expression of genes was not related to age, sex, size, and tumor location (p > 0.05).
Dysexpression of lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 can be used for diagnosing OSCC.
口腔鳞状细胞癌（OSCC）是全球范围内一个重要的健康问题，长链非编码RNA（lncRNA）的表达在这些恶性肿瘤中发生改变。本研究评估了伊朗德黑兰阿米尔·阿拉姆医院转诊患者的口腔和咽部鳞状细胞癌（SCC）样本石蜡块中ATXN1、CDC42EP1基因以及与这些基因相关的lncRNA（lnc-ATXN1L、lnc-ATXN1、lnc-ATXN10和lnc-CDC42EP1）的表达水平。
本横断面研究对德黑兰阿米尔·阿拉姆医院转诊患者的76份口腔和咽部鳞状细胞癌（SCC）样本石蜡块进行。使用qPCR Master Mix试剂盒在所有样本中测量ATXN1、CDC42EP1、lnc-ATXN1L、lnc-ATXN1、lnc-ATXN10和lnc-CDC42EP1的表达水平。采用实时PCR进行反应，以甘油醛-3-磷酸脱氢酶（GAPDH）作为管家基因。利用社会科学统计软件包（SPSS）22.0版进行统计分析。两组之间lnc-ATXN1、lnc-ATXN10和lnc-CDC42EP1的表达存在显著差异。它们均下调（p < 0.05），其他基因在SCC样本和相邻组织之间未观察到显著差异（p > 0.05）。基因表达与年龄、性别、大小和肿瘤位置无关（p > 0.05）。
lnc-ATXN1、lnc-ATXN10和lnc-CDC42EP1的表达失调可用于诊断OSCC。
